FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1969-lungUR-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1969-lungUR-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541196
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG5980.11049601253519982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACT1401.4124453E-841.4284674
GTTACGT800.002032125436.263311
CTATTGG1102.429437E-432.966651
CCACGAA1801.290573E-732.2221372
CTAATAC1352.1404347E-532.2221373
TAGTACC1453.4875266E-529.9999249
GTATTAT3650.029.8054621
ACGAACC2156.115988E-726.9766754
TATACTG1658.411711E-526.363575
CAATATA1409.988798E-425.8927924
CGAACCC2301.1015745E-625.2173255
ATTATAG1751.2547064E-424.857083
GTTGACA5350.024.4014781
ATTACCT4850.023.9174654
GTCCTAT7000.023.8301751
ATACTGT3052.030356E-823.7704326
TCCTATG6750.023.629572
CTATGAT7550.023.0462994
TATAACC1902.1914004E-422.8946783
ACCTCTA5450.022.614627
Produced by FastQC (version 0.11.5)