FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1969-lungUL-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1969-lungUL-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823539
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAGACCTTGTAGTTTCTACTGATAGCTTCTATTCTCAGTGAAATGG17150.20824757540322922No Hit
ATTTTAGAGTGATCGCTTACACTGAAAAATATTTTGGCCTAAGTTTCATA10420.12652709829164133No Hit
AAATAAGGTAGTGCATGGGCTCTGAAGAAACAGTTTATACCCAGTTAGGT9730.11814862441244434No Hit
GCATATACAAAACTGAATTCTTGTTCTTCCCCCAAATATCTGCAGAATTC9040.10977015053324736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG2950.049.1523749
TTAGAGT3000.048.3361054
AGTGATC3500.045.5712748
GCTATAT2700.042.9706461
CTATATA3000.041.085692
TATATAC4650.038.9807323
TATACTG3600.036.252085
TACCTCG850.002741750334.117535145
GTATGAA3650.033.7731631
TAGAGTG4550.033.463465
CGAAAGC4950.032.224073
TATACCA7950.031.920075
ACTCCGC1652.3858156E-630.7574738
ATATACC6050.029.9603984
AGACGGT3900.029.743492145
GCATATA7750.027.1338831
TGGAGCG1358.078787E-426.8533945
TAGTATA2456.4319465E-826.6341822
GAGTGAT5200.026.4902957
TATTCGA550.002636994726.3651472
Produced by FastQC (version 0.11.5)