FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1969-lungMR-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1969-lungMR-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences657135
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG1800.048.333269
TATACTG3850.033.8960535
GCGAAAG3350.032.4626352
GTATAAG1153.156727E-431.5288871
AGCGAAA3500.031.0784741
CTATATA1753.772071E-628.9999542
ATACTGC2903.947207E-1027.4999566
GTATTAT6350.026.2657991
CGAAAGC4150.026.2047793
TTAGATA1409.991027E-425.8928154
GTATTAG1751.253154E-424.862781
GTTATAG1751.2550985E-424.8571033
TCGCCAT2351.3292974E-624.680813145
ATACTGT5350.023.0373466
TAGAGTG4455.456968E-1222.8089525
CATAAGA3503.4960976E-922.7856794
AGTGATC4850.022.4226468
TATAATA4557.2759576E-1222.3076572
GTTGACA5550.022.2122441
ATTGGAC1650.002599942521.9696643
Produced by FastQC (version 0.11.5)