FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1969-lungLR-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1969-lungLR-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937270
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG13230.1411546299358776No Hit
GTCTGATATTCCTCTACATGGAGAAGAACCAGCTCGAAGAGGTGCCCTCG11510.12280346111579374No Hit
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC11470.12237668974788482No Hit
CCCTTGAAGTAGCCGTTCGGGATGGTCTCGATCCTGTTGCTGTCCAGGTA10330.11021370576248041No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC10070.10743969187107236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAT2050.053.05441
CTATATA1800.052.361072
TATACTG3750.034.7999735
TAGTATA1051.8536356E-434.5237852
CTTCGAC1102.4360434E-432.954523
ATATACC2653.6379788E-1232.8301664
AGACGGT2950.031.94913145
TATATAC3300.030.7575533
CTACTGT7150.030.4228041
TTCGACA1255.1536184E-428.9999794
GTAATAT2553.0195224E-928.4343851
AATAGAC1855.8156584E-627.4324113
TACCATG4400.026.3636177
AGAGCGT1100.00967131526.3636178
AGCGAAA3607.2759576E-1226.1833291
GTATATA1958.746523E-626.0283991
CCTTCGA1409.994166E-425.8928382
CATATTG1409.994166E-425.8928385
TACTGTG9150.025.3551712
TATAACA2351.3302979E-624.6808325
Produced by FastQC (version 0.11.5)