FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1969-lungLL-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1969-lungLL-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1110071
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG14650.13197354043119766No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC12670.1141368434991996No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT12000.10810119352726087No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATCG350.003566624662.142783
GCGATTA1801.2926284E-732.222183145
GTTACGT1402.7435897E-531.076991
TATACTG4500.028.9999665
GTCGAAG1255.154239E-428.9999643
TTACGTG1907.15643E-626.7104932
TCGGAGC1409.995359E-425.8928268
GTTATGT5800.025.0044751
CTACTGA15400.024.9512656
TACTGAA15200.024.80267
TACGTGT1902.1933981E-422.8947093
ACTGAAC17850.022.7450718
TAGTCGG2552.7075603E-622.745078
AGTCGAA1600.00217531522.6562232
GTTGACA10050.022.3671881
ATTAACG1650.002601057521.969673
ATCTCGC2353.7045364E-521.599611
GTGTAGG5101.8189894E-1221.3273451
TAGTACC5950.020.714269
ACATGGT11000.020.4317935
Produced by FastQC (version 0.11.5)