FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1968_lungUR_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1968_lungUR_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences938890
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG9560.1018223647072607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAT2151.2732926E-1137.100791
GTATATC1803.1923264E-936.257591
ATATACC2800.033.662384
TATACTG2653.6379788E-1232.8318145
TAGTCGG1652.3862922E-630.7574638
GCATTAT1255.147291E-429.0060711
CTATATA2853.2196112E-1027.9853322
GTGCGAA1306.4807734E-427.8859984
TCTACGG1050.00770635227.6204173
GTTGACA9000.026.5888981
TATATAG2259.102587E-725.7790575
GTTATGT4800.025.6824591
GTAATAT2559.465657E-825.5935941
TATTGGG5100.025.5908662
ATACTGT5150.025.3410646
TCATAAG4350.025.0012423
ACATGGT9450.024.5514835
GTATTAT9750.024.54361
TATTATA3001.7049388E-824.1691512
TATATAC3902.0008883E-1124.1678643
Produced by FastQC (version 0.11.5)