FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1968_lungUL_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1968_lungUL_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1259
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATTCCCTGAAACCATTGATATGCAAAGGCTCTTAAGAATGTACTATG30.23828435266084197No Hit
GATACTTGGGTGAGGATTTTTATGTTTATGTTCATCACAGATACTTGGGA20.15885623510722796No Hit
GAACCCTCCATCCAAAAGCAGCAAAAGGAATATACTCCTCTCAAGTGCCC20.15885623510722796No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC20.15885623510722796No Hit
CTTTAAAAAAATAAAGTGAGCTCTATGCCCAGTGTGGGGTTGAAACTCAA20.15885623510722796No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT20.15885623510722796No Hit
ACCTACTAAGATTGAATTCTAAAGAAACAGACATCTGACTGTTCAGCTGT20.15885623510722796No Hit
CCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAGAA20.15885623510722796No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG20.15885623510722796No Hit
AGTCAGTGGAGCTCTGTGACTCTTGATCTTGTGGTTTTGAGGTTCAACCC20.15885623510722796No Hit
CCTGCAGGGAGGAGACACAGGAAGTTACTAGCCTGCTGGACCTCTGGAGT20.15885623510722796No Hit
AAAATACAGAGTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAG20.15885623510722796No Hit
AGTCAGTGGAGCTCTGTGACTCCTGATCTTGTGGTTTTGAGTTTCAACCC20.15885623510722796No Hit
TCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCA20.15885623510722796No Hit
GTTCATGGGCAAACCAAAATAGGAACACAGAAATACTTTGAGGTAAATGA20.15885623510722796No Hit
CTTTAATACCTCACACTTCCCTCAATGATCCCCCATCCAGACAGAAGATC20.15885623510722796No Hit
AGCCAAGCCTGGGCCTATGGGAGCCTTTCTCAGTCCACATTAAACTCAGA20.15885623510722796No Hit
GGGTGTGGGGACCAGCCAGGCGGCAGAAGCACAGGTCGGGGTGGCAGTGA20.15885623510722796No Hit
CACTTTATTTTTTTAAAGATGTCATTTATTTATTTGACACAGAGCTGGAG20.15885623510722796No Hit
CATATGACCTAAGGTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATAT20.15885623510722796No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT20.15885623510722796No Hit
GCCCTGCACCAGGCTCCACACTCAGCGGGGATGCTGCTGGAGGATACCCT20.15885623510722796No Hit
GGGCCTGGGCCTGGGTCTTGGTCGCCTTGTCAGCTGGCCTTGGGCCACTG20.15885623510722796No Hit
TCTAGGAACAGGATGATTTTCTCACGCTGTAAGTGGGGAGACCAAGCACT20.15885623510722796No Hit
CACAGAGCCTGACTCCATCCAACCATCTTTTCCTGGGTTGGTAGCCCTGC20.15885623510722796No Hit
CTGCGGCAGCGTGCGTGGCCTAGGATGGCACTCTGCTCACAATGCCATCT20.15885623510722796No Hit
GAATCTGGGCTTGAAGGGTGGATTTCAAATCATTTTTAACAACACGGCAC20.15885623510722796No Hit
TATATGCTCAAGGGAGGACGCACATAAGGGCAGAGTAACACACGCAAAAC20.15885623510722796No Hit
GAGCGTGTCTACAGCATTTTCCGTTCTTTCCAGGACCTCCGCAAGCCCTG20.15885623510722796No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)