FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1968_lungLR_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1968_lungLR_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1771
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC50.282326369282891No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA30.16939582156973462No Hit
GTCTGGGCCTGGGCCTGGGTCTTGGTCGCCTTGTCAGCTGGCCTTGGGCC30.16939582156973462No Hit
GTGTACTATGTACCCATTCCTTTAGGTAGAGCCTTTAATACCCACTCCCT30.16939582156973462No Hit
CTGACAGACTGAGCAAAGTGGAAGGAGCAGCAGGCAGTAGAAGAGGGAGA30.16939582156973462No Hit
GTCCCTGGAAGTGCGTGACTTGGTGCACGAACAGGCTTGTTATGGAAATA20.1129305477131564No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT20.1129305477131564No Hit
ACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAGTCCTTC20.1129305477131564No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG20.1129305477131564No Hit
AGAGAATAGTGTCAGCAAGTTTTCTGAAAACGATTGTGAAACTGCTTTTT20.1129305477131564No Hit
AGCATATATGAACAAACCTAAGAGAAGCAATCAGCACCAATACCATAGGG20.1129305477131564No Hit
GGTTTCACCTGTGAATTCTACGAATCATCCACAGAAAAAAATGAATACCA20.1129305477131564No Hit
AAATTAATACAATGAAACAAAATTCACAGAATGCAGTAAAACAGGTTCTA20.1129305477131564No Hit
TTTTCAAAGATGCCATTTATTTATTTGACACAGAGCTGGGGAGAGAGACA20.1129305477131564No Hit
GTATAAAAGTGCTTTATAAACTAGGAGATACTGCCCAATTATGAAAATGA20.1129305477131564No Hit
GGGACTTGCTGGAGAAGACCCTTAGCCTCTCAAGTGCTTGGTCTCCCCAC20.1129305477131564No Hit
CCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTT20.1129305477131564No Hit
CTGACAGCCTGTTGTAGTTCAGGCGAATGAAGGCAAGGTTGGGGAAACCC20.1129305477131564No Hit
GTTACTAGCCTGCTGGACCTCTGGAGTTAGAGGCCCTGTCTTGGCACAAG20.1129305477131564No Hit
GGGATGACCTCATGGTCCAGGTGACACACCTGCAGGGAGGAGACACAGGA20.1129305477131564No Hit
GTCTGATATTCCTCTACATGGAGAAGAACCAGCTCGAAGAGGTGCCCTCG20.1129305477131564No Hit
CAATAGATTCCTCGCCTGGGCAAGCCACTTCCTCCCTCCAGGACCCCCCA20.1129305477131564No Hit
GGATTAGGTAGGAAGAGACTTGGAACATAGAAACTTAAAGAAAATCTTGG20.1129305477131564No Hit
GAGTTAGAGGCCCTGTCTTGGCACAAGAGTTGGCCAGGATGGGCTCCAGG20.1129305477131564No Hit
TGTTGAAGGAGTTCTTGGGGAGCCCCCGGTCTGACAGCCTGTTGTAGTTC20.1129305477131564No Hit
GGATCATTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGTA20.1129305477131564No Hit
CCAAGGTGGTGATCAATCTGTCAGCCTGTTGTCTTCAGGGACCTGCCATT20.1129305477131564No Hit
GTACACATCCAGGGGCCACTTGGGTGAAGGCAGAGCACGGGCACTCCAGC20.1129305477131564No Hit
GTATTTCTGTGTTCCTATTTTGGTTTGCCCATGAACCCAATTGGTTGTTC20.1129305477131564No Hit
CTGTTTAGTGTTAGTATCTAGACAAGGAGCTGGCAGCAAAGTTCCTGTTT20.1129305477131564No Hit
GGTTTGCTGTTCAATAGATTCCTCGCCTGGGCAAGCCACTTCCTCCCTCC20.1129305477131564No Hit
GTTGTCTTCAGGGACCTGCCATTTAGGAAAACAGGTGAAAGTTGGATTTA20.1129305477131564No Hit
GGCCTGAGTTTGGGTCTGGGCCTGGGCCTGGGTCTTGGTCGCCTTGTCAG20.1129305477131564No Hit
CATTAGCTGAGTGTGCCAGGGAGCGGGAACAGTGTGGCTTGGGCAATTCT20.1129305477131564No Hit
TCACAGAATCTGGGCTTGAAGGGTGGATTTCAAATCATTTTTAACAACAC20.1129305477131564No Hit
GGATAACAACCGGATTCGCAAGATAGACCAGAAGGTTCTGGAGAAACTGC20.1129305477131564No Hit
GAATAAACATTGAGTGACTAAACACCACCAAGGTGGTGATCAATCTGTCA20.1129305477131564No Hit
GAGAAGAACCAGCTCGAAGAGGTGCCCTCGGCCCTGCCCCGGCACCTGGA20.1129305477131564No Hit
CAAATAAGCAACCTAACTTGATACCTCAAGGAAACAGAAAAGAACAAATA20.1129305477131564No Hit
TGGTTTCACCTGTGAATTCTATGAATCATCCACAGAAAAAAAATGAATAC20.1129305477131564No Hit
GTTATGGTCCATTTTGGTCTGAACACCTTTGTAAGCCCTACAGTTCCAGA20.1129305477131564No Hit
CTTGTCGGCCTGTACCCAGCAAGTAAGAAAATAACATGGACTTGAGGGTC20.1129305477131564No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG20.1129305477131564No Hit
GAGCAGGGGTGTGCTTTGTTCATGGCTGCAGAACTAACAGATGATCTTCT20.1129305477131564No Hit
CTAGAATCCCATAACAGAGGCCGTTGGAGCCAGAAGTGCTTCAGACTTGA20.1129305477131564No Hit
GATTGAATCAGATAAATTTTTACACTCTAAAAGAGTAAAGTACAGAAAAT20.1129305477131564No Hit
ATCCTGTTGCTGTCCAGGTAGAGCTGGTGAATGGCCGTGGGCACCTTGGG20.1129305477131564No Hit
CTTCATAACGGAGCTCCCCGTGGAGTCCTTCCAGAATGCCACCGGCCTGA20.1129305477131564No Hit
GTGCAGTGATCCTCTCGCGTCTCCTCATGGCTGGGGAAGGCACTGGCCTA20.1129305477131564No Hit
AATATGTAGAGCATATATGAACAAACCTAAGAGAAGCAATCAGCACCAAT20.1129305477131564No Hit
ATCACAGATACTTGGGATGTCTCGATAAGGGCCGTGCTGGCCTCATAAAA20.1129305477131564No Hit
CAAGACAGACAGTATGGTTATGGTTATGATTTAGAACGCGGCCTTTAGGG20.1129305477131564No Hit
GAATCTGGGCTTGAAGGGTGGATTTCAAATCATTTTTAACAACACGGCAC20.1129305477131564No Hit
CCCTTAAGCATCTTGCAAGATGTAAGACACAGCCCCCTTGCTCCATCCTT20.1129305477131564No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACAGA100.0065013645145.06
Produced by FastQC (version 0.11.5)