FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1968_lungLL_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1968_lungLL_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834856
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12400.14852860852650038No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC11490.13762852515882978No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT8820.10564696187126883No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGG604.94467E-448.333217
AACGGCG855.3408112E-542.646954145
GTATTAT5300.034.2103231
GTTACGT850.00273693334.1298261
TAGTACC2653.6379788E-1232.830119
GTACATA900.003621554532.2337271
GTATAGT1352.1365595E-532.2337271
GCTACTA1652.385852E-630.75759
CCTATAT1555.4859887E-528.0745351
TGCTCGC1306.482141E-427.884544145
CTATATA1855.7985126E-627.4422251
TACATAG1855.81474E-627.4323645
TATTATT7150.027.3775542
GTCCTAC2654.5383786E-927.368261
TAGAATA4150.026.2047544
GAATAAC4750.025.956631
GTTGACA7850.025.8691041
GTATAAG2259.076193E-725.7869781
TATACTG2259.104697E-725.7777125
GTATATA1801.5163158E-424.1752951
Produced by FastQC (version 0.11.5)