FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1968-lungUR-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1968-lungUR-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences843155
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12460.14777828513144084No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC11640.13805290842134602No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT8920.10579312226103148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAA2000.043.5023844
GTAATAG1157.138693E-637.828163
TTAGGTA1652.3848224E-630.759264
TATACTG1652.3848224E-630.759265
CTACTGT4750.029.0084721
GTATTAT7300.028.8097821
GATATAT2553.0122465E-928.4396761
GTAAGAC2054.0359555E-728.2942333
TAGCCTA1555.4973596E-528.0660535
GTTATGT3255.0931703E-1126.777051
CTATATA2207.476174E-726.3666482
TATCCGG1100.00967095126.363518145
CTACTGA5550.026.1275586
GTACTGG2808.167262E-925.900421
CCTATAT1409.97627E-425.900421
TGTGCGA3657.2759576E-1225.8233323
CTGTGCG3950.025.6991352
GTATATA2559.4556526E-825.5957091
TATTATT8000.025.3778952
GTATACG1450.001224197625.0073011
Produced by FastQC (version 0.11.5)