FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1968-lungUL-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1968-lungUL-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1069435
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG18300.17111839429231324No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT17030.15924296474306526No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC13150.12296212486032343No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT12300.11501400272106298No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG12090.11305034901606924No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA11330.10594379275037753No Hit
CTTTAATACCTCACACTTCTCTCAATGATCCCCCATCCAGACAGAAGATC11280.10547625615395044No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC300.001932952872.52671
GTTACGT1150.069.373371
TTACGTG1500.053.171332
GCTACTA1900.045.789219
ATATAAC3400.040.518262
TAACCTA3550.038.806225
GTATATC2650.038.3159981
GTTATGT4750.038.171951
TACGTGT2109.094947E-1237.9795233
TATAACC3750.036.7365573
ACGTGTT2201.6370905E-1136.2531784
TACTTAG5100.034.1174477
TATACTG3450.033.6261375
CTACATC3950.033.0408743
CCTGTTA4200.032.8004952
ATAACCT4800.031.721534
TTAGGTA2151.8091669E-830.35154
CTGTTAC4600.029.9482783
CGGACCG1000.006072040629.0025445
GTGCGAA1504.396359E-529.0025424