FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1968-lungMR-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1968-lungMR-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1555158
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG25260.16242722604391321No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG23690.1523317887957365No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT21160.13606334533211417No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC19210.12352442645699022No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC18520.11908757823963867No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA17150.11027818395301313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1801.8189894E-1244.315311
TATACGG951.02859965E-438.161395
TATACTG7100.036.763935
GTATATC4800.034.7472341
GTGCGAA1905.4260454E-934.345254
GTTATGT9950.034.2537731
GATATAC4050.034.015463
TTACGTG2507.094059E-1131.9029222
TATATCC5350.031.1710782
GGATATA4900.028.1148192
CGGACCG1050.007706301327.6215765
GACGATG3800.026.710394145
TTTTGAC7150.026.366052
ATCGTGC1100.00967343626.3635068
TTATACG1409.991752E-425.895234
CATTCTA16900.025.7420022
TGCGTTA4250.025.5881128
TAACCTA4101.8189894E-1224.7583665
TTATGTG13850.024.6051432
GTTGACA19750.024.2331851
Produced by FastQC (version 0.11.5)