FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1968-lungLR-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1968-lungLR-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1105208
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG15990.1446786487249459No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT15330.13870692213592373No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC14130.12784923742861073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1300.072.5161741
TTACGTG1850.050.948092
GTTGACA11950.037.6234551
TATCGGC800.002036738436.249897
GTGATCG1001.3915694E-436.2498869
GCTACTA2551.8189894E-1234.1175429
ACATGGT13400.032.4640545
ACGGGAT900.00362764732.223583
GAATAAC7900.032.127421
GTTATGT4700.030.8579461
GTATTAT12850.030.4737261
TACGTGT3201.8189894E-1229.4543673
TTGACAT15850.027.9033832
CGTGTTG3453.6379788E-1227.3199925
GCGTTAA1606.82892E-527.1874169
ATATACC2705.5624696E-926.8529834
TACACCG3255.0931703E-1126.7703595
CAGCAAC7050.026.7375077
TTATACG1100.00967003226.3647464
GCAGTGC10200.025.5939461
Produced by FastQC (version 0.11.5)