FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1968-lungLL-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1968-lungLL-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948329
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG9600.10123069103654955No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC803.726589E-545.3287851
CCGTACA803.7300735E-545.3216132
GTTACGT951.0266759E-438.1716081
GCTACTA1553.470268E-837.4189879
GTTATGT3700.031.362621
TATACTG2701.70985E-1029.5429785
TATAACC2802.6193447E-1028.4878713
GATATAC1050.007700690527.6246033
ACTATAC1358.082865E-426.8515878
TATAGGT1100.00966185926.3689422
TAACGCA1658.406611E-526.368945
AGCGAAA4450.026.0767841
CTGATCG1701.0313062E-425.5879849
GTAGGCT2601.1423981E-725.10126
AGCTATA1450.00122646724.9997547
GTATTAG2351.3261597E-624.689721
TCAATAC1500.001492467424.1715263
GTGTTAC4550.023.9096871
ATAATGC1851.8280756E-423.5182423
GCGAAAG5250.023.4809132
Produced by FastQC (version 0.11.5)