FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1967_d08_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1967_d08_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3317
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA70.21103406692794696No Hit
GAAAAGAGGAGAGAGGAATGGAGTCAGCCCGTGAGGTGGGGGGATTGCAC50.1507386192342478No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT40.12059089538739826No Hit
GTTGGGAGGTTGGGGTTCTGGGTGTGGGACGAGGAGTGGGGTAAGAGGCA40.12059089538739826No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG40.12059089538739826No Hit
CTTTTATACACATCCTCGTATTGGAGTCAGGATATGGTCATTGGAGAAGA40.12059089538739826No Hit
CCATAGCACCAGGCCCGCGAGACACTCCTGAGCCCCCGTCACTAGGTCAC40.12059089538739826No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG40.12059089538739826No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGAGTG100.0067759887145.05
Produced by FastQC (version 0.11.5)