FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1967_d04_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1967_d04_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1866
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA50.2679528403001072No Hit
CTTATATGGACTTGACAGATACACACAGAACCCTCCATCCAAAAGCAGCA40.21436227224008575No Hit
CTGTGACTCCTGATCTTGTGGTTTTGAGTTTCAACCCCACACTGGGCATA30.1607717041800643No Hit
TCTATTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCA30.1607717041800643No Hit
GTTGACATGGTCCTATGATGTTGATCCTTTGTTTTGTGAATGCGGCACAT30.1607717041800643No Hit
GTAGTATGTAGGCCATTTCCCAGTAGTGTGACCCCACTTTCCATAGCTGG30.1607717041800643No Hit
GCCCTGCACCAGGCTCCACACTCAGCGGGGATGCTGCTGGAGGATACCCT30.1607717041800643No Hit
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC30.1607717041800643No Hit
ACCGTATGATGGGAAGCCACAGAGACAGGATGACAGGGTTTGATCTGGAA20.10718113612004287No Hit
CAGCCAGGCGGCAGAAGCACAGGTCGGGGTGGCAGTGATTTGGTAGCAGC20.10718113612004287No Hit
CCCTCCGGCCTCCTTCCTCATCCGCAGTCTTCTCTTGAGTTAATGGCCAG20.10718113612004287No Hit
GCTCTGGTGAGGGTGAGCCATCCTGGCAGAAGGCACACATCAGCTGTGAC20.10718113612004287No Hit
GTAGCATTTGGTTTGCAGATACTTGGGTGAGGATTTTTATGTTTATGTTC20.10718113612004287No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT20.10718113612004287No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG20.10718113612004287No Hit
GTTGTAGTTCAGGCGAATGAAGGCAAGGTTGGGGAAACCCTTGAAGTAGC20.10718113612004287No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC20.10718113612004287No Hit
ATGTAGGCCATTTCCCAGTAGTGTGACCCCACTTTCCATAGCTGGAGAAT20.10718113612004287No Hit
GGTGAAAGTTGGATTTATTCATTCAGTTAGTCACACAATGATTTTCCCTA20.10718113612004287No Hit
GTATAGACACATGACACTTAAACCAATTTTCTATCATTGGATTTTTCAAT20.10718113612004287No Hit
GTTCTACTCCATTGGAACTGAAAATAAGAAGATCATCTGTTAGTTCTGCA20.10718113612004287No Hit
GTAATATACTGTGCTGCTTCTGAGAAACCAGCAGGGCCTGTTAATTCTTT20.10718113612004287No Hit
GTGTATGAGTGTACTGTTTTATGCAGTGGTCCTGACTCAGTTAGTGGGAT20.10718113612004287No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA20.10718113612004287No Hit
GAACAAGCGTTGCACGCTTTACCAACTGAGCCAGCCAATACCAGTACCAA20.10718113612004287No Hit
GAAAAAGACATCTGAACAGACTAATGACCAGTAGAGAGATGAAATAAGAA20.10718113612004287No Hit
GTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCTACATGCACAA20.10718113612004287No Hit
GACCTAAGGTCCAGCCAGATCTCCAGGTAAGGGAGGGAGAGAGGACATGA20.10718113612004287No Hit
GGATCATTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGTA20.10718113612004287No Hit
GCTTAAGATTCTCTCTCTCTCCCTCTGCCCCTCCCCACTCGCTCTCTTTA20.10718113612004287No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT20.10718113612004287No Hit
GCATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC20.10718113612004287No Hit
GAAAACAAGAAGATCATCTGTTAGTTCTGCAGCCATGAACAAAGCACACC20.10718113612004287No Hit
GTGTATGTGTGTGTACGCACGCCATTTCTTCTTTATCTCCTCTGTGGCAG20.10718113612004287No Hit
TTCCATAGCTGGAGAATGTATTCATCACTATTCCCATGTCATGGGGTCGG20.10718113612004287No Hit
GTTAGGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG20.10718113612004287No Hit
ATAATAAATAGGAAACACTCGAGGTTCACTACAATTTGTCCTTATTCCAA20.10718113612004287No Hit
GAGCAGGGACTCCAACGTGGGGCTCGATCCCAGGACCCTGGGATTATGAC20.10718113612004287No Hit
GGTCAGGGGGCAGTGATTTGGTAGCAGCGCTATCAAATGCTTTTTCTGCA20.10718113612004287No Hit
ACCCAGCATCGTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATC20.10718113612004287No Hit
CTTCCCTCAATGATCCCCCATCCAGACAGAAGATCACAACGGAAACATCA20.10718113612004287No Hit
GAATGTATTCATCACTATTCCCATGTCATGGGGTCGGTGAGCATGACCCA20.10718113612004287No Hit
GATTTCTGTTTCTTTAGGATTCAATCTTGGCAGGTTGTATGTTTCTAGAA20.10718113612004287No Hit
GGCTCTGAAGAAACAGTTTATACCCAGTTAGGTGGGAGGAAATGGGAAAA20.10718113612004287No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC20.10718113612004287No Hit
TCCTACCACTAGGCCGAGAGGGGCTGCATCCAGGGACTCAGAGGCTGATA20.10718113612004287No Hit
CTGCTGAGCAGAGAACCCTATGCGGGGCTTGATCCCAGGACCCTGGGATC20.10718113612004287No Hit
GCCCAACACAGGGCTCCATCACAGGACCCTGGGATCATGACCTAAGCCCA20.10718113612004287No Hit
ACCCCACACTGGGCATAGAGCTCACTTTATTTTTTTAAAGATGTCATTTA20.10718113612004287No Hit
CCTCAAATCAGAAGTGATTAAAACACAGCCACACTGACTCTGAATTATGT20.10718113612004287No Hit
GCGTTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGTATTC20.10718113612004287No Hit
ATAGAATCCAGTTCCATTTGTGCCCCCAGCTCATTCAGTAACGTTGGGCA20.10718113612004287No Hit
GTCCACCAAACACCCCGAATTCAAGGAGATCCAAATCGAAGTTATGATTG20.10718113612004287No Hit
CCTCCATAGGCTTCACAGCCCCCCTTTATCTGGATTGCCAGTTCAGGAAG20.10718113612004287No Hit
GCTCTTAAGAATGTACTATGAACAATTACACCCCAACAAGCTGGATAATC20.10718113612004287No Hit
ACATCAGGAGGTTCCATCCAAAGTTCAAGGTTTCTCCTTCCATGAAATGT20.10718113612004287No Hit
TCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCA20.10718113612004287No Hit
AGCCTAGGCTGGAGGTGTGACCTCCCCGCCGGAGGCTACAAATGGACGAG20.10718113612004287No Hit
AGGTAGTATGTAGGCCATTTCCCAGTAGTGTGACCCCACTTTCCATAGCT20.10718113612004287No Hit
CAGGAGAGCCGGCCCTGGCAGGAACCGCGGGCCGGTGGCAAGTGTCACCC20.10718113612004287No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT20.10718113612004287No Hit
GTATTTGTCTTTATGCCTCTGCAGTGGTAGGACGGTTTTTCATGGAGGAA20.10718113612004287No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG20.10718113612004287No Hit
GTGGTAGGACGGTTTTTCATGGAGGAAGGGGCCCACAAAGGCAAACATGC20.10718113612004287No Hit
GGCTCAAGGATCGGGTCCTGAGACCACCAACATCAGGAGGTTCCATCCAA20.10718113612004287No Hit
CCCCAGACCCCCGTGTCACCTGCCTTATCTCCAGTTCTCTGGTTCATGGC20.10718113612004287No Hit
GCCCATGCACTACCTTATTTAGTTTGTAAACATAATTCAGAGTCAGTGTG20.10718113612004287No Hit
GGATCACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCAT20.10718113612004287No Hit
CCTAGAACGTTCTCCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCA20.10718113612004287No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)