FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1967-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1967-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542447
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACTGTGCTGCTTCTGAGAAACCAGCAGGGCCTGTTAATTCTTT12180.2245380654699906No Hit
CTTATAAGGGGTCGAGTATTCATTACCACAATTCAACAACAAAAAGACAA9070.1672052753540899No Hit
GTGTAAGAGAGTGTGTCTAGTTTCATTCTTCTATATATAGTCACCCAATT7680.14158065211900886No Hit
GTATTTACATGTGTAATCACCTTCCAGATCATGATATAGAATACTTAAGT7250.13365361039880394No Hit
CTTTTATAATATGCAATGTGAGGTCATCGCTGTTCACTAGAAAGTGGAGA7220.13310056097646406No Hit
GCATTATCCAGCTCCAGAATTTTAGCCACGACTCCCTGTTTCAGGTGACT6780.12498916944881251No Hit
TCCCTATACTGTCTCTTTCTGGATGTTCAGACACAGTGATCTATCTGAGG6150.11337513157967506No Hit
CTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCAT5950.10968813543074254No Hit
GAGTAGCATTATCCAGCTCCAGAATTTTAGCCACGACTCCCTGTTTCAGG5740.10581678947436338No Hit
GGTCTACATCTCCTCCAGGCTATATCTTCTACGGGATCTCAGACAAGGAC5530.10194544351798424No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGC301.5981099E-596.6750262
TCGTATA406.660629E-572.49958145
TAACTAA502.0085617E-458.0050135
TAGTTAC553.2163132E-452.7318343
TGATCCG553.2177777E-452.726979
TACATGC1602.0008883E-1145.312247
TTGAGCG803.732505E-545.312249
CAGTGCG657.333809E-444.615139
TATACTG9550.042.5167665
CCCTATA2250.041.892512
TCCTATG2100.041.4321562
TAGTGTA700.001055763841.432154
ACACCGA1807.094059E-1140.281266
ACCGACC1701.8262654E-938.3821338
ATATACT7250.038.0032844
CCGACCT1752.4247129E-937.28559
TACACCG1803.1923264E-936.2531365
GGGATAC4600.036.249794145
CACCATA3400.036.249797
GGGTCGA2650.035.5658349
Produced by FastQC (version 0.11.5)