FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1967-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1967-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853422
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAATATGCATGACCTTGTTTAGCCTTGCAATGGCCTTGTAGAGTTG20990.24595100665321495No Hit
GTCCTGGAGTGACCCAAGACAGGGATGGGAAACAGTGGGTGACCACACAT12360.14482870139274592No Hit
TAATAGTACTTACTGCAGTAGGCTGAGGTCAGGGATGAAATGAATTGGGA8950.10487191565251423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG3100.063.155652
TCGTTAC702.3314533E-762.153183
AGCGACA350.003566461862.1422548
CTTATAG2350.061.7123763
ATACTCG606.798042E-660.4267045
CGAAAGC4000.050.7584273
AGCGAAA4200.048.3583641
GTATATA2450.047.3714561
GTGATCG2200.046.1359189
CCAATCG700.001055690941.435453
CTAATAC1053.8190974E-641.435453
CAATCGG1105.2573923E-639.5520254
TCCGGAT2057.2759576E-1238.9088973
CCGGATC2057.2759576E-1238.9088974
ATAGAGC4350.038.33976
TGGATCG1157.1333434E-637.8323675
TATAGAG4250.037.535655
TCTAATA1209.551322E-636.2560232
TTATAGA4500.035.4503334
TATATAC1656.006303E-835.1573523
Produced by FastQC (version 0.11.5)