FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1967-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1967-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705555
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG13410.1900631417820014No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC12100.17149619802850238No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA9350.13251978938566095No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT9230.1308190006448824No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA8570.12146466257060046No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG7930.11239378928644826No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT7590.1075748878542424No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGCG800.002036219736.249969
GTATTAT7700.035.784261
GTAGCAT14950.033.9512671
GTTGACA5850.030.9872681
GCATGTT13450.029.646814
ATGTTGT17250.029.4202636
CCGACTA1753.772573E-628.999974
GGCGTCG1050.00770697527.619022145
TTGTTTA17250.027.3188159
TTGACAT6800.026.6543872
CATGTTG12850.026.5174855
TAGCATG15350.026.4494842
ATCCGCT1100.00966973726.363611145
GTATATA3501.2914825E-1024.8606411
AGCATGT16300.024.4631653
GTATTAG2101.5525318E-524.1700691
TATATCT3001.7053026E-824.1666434
TAGTTGT2101.5542373E-524.1666434
TGTTGTT21150.023.652467
GTTGTTT20250.023.6296068
Produced by FastQC (version 0.11.5)