FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1967-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1967-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471739
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC5800.12294934275097036No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG5750.1218894346238068No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGCGA450.009636248.3330848
CTATTAC657.3251576E-444.6246151
GTTACGT750.001481083938.6746671
GCGAAAG1752.421075E-937.289482
AGACGGT1209.55348E-636.249813145
AGCGAAA1905.4023985E-934.3492131
GTATTAT4300.032.0415151
GCTATAT1153.155754E-431.5282611
TATACTG2202.2566383E-829.6620855
CTAGACC1000.006068255329.0029263
TACATAG1255.1467237E-429.0029245
CAGTGCA4100.028.2955362
GTAGAAA3102.7284841E-1128.0673472
TTACGTG1050.007700598327.6218362
CGAAAGC2405.254151E-827.190243
GGGTGCG1100.00966674726.36357
ACCGTGC1100.00966674726.36358
GCAGTGC4500.025.7831121
CTACATC2601.1409611E-725.0986843
CTATATA2051.2873772E-524.7585952
Produced by FastQC (version 0.11.5)