FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1966_lungUL_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1966_lungUL_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872121
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG10060.11535096620767073No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG9120.10457264530953847No Hit
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC9100.10434331933298246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT858.9199784E-751.1793821
GTATATC1255.1932147E-946.402641
CTATATA1755.2750693E-1141.4285552
GCATTAT951.028438E-438.1600651
ATATACC1553.4695404E-837.419344
CGGACCG1102.4359014E-432.9545335
GCTATAT1902.0750304E-730.5280531
GGTTATA1902.0750304E-730.5280531
GCCTATA1453.4885314E-530.0017091
GTTATAT1504.398083E-528.9999892
TATATCC2252.8161594E-828.9999872
GTATTAG1804.6969762E-628.196051
AGACGGT2104.9852315E-727.619036145
TTAGGTA1855.8150345E-627.432424
CCGACTA1606.827171E-527.1874894
GTATACC1100.009668237526.3651371
TTACGTG1658.417382E-526.3636262
GTTATGT4150.026.2063121
CTTATGA1701.0307455E-425.5896911
GCGAAAG4550.025.4959561
Produced by FastQC (version 0.11.5)