FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1966_lungLR_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1966_lungLR_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAGGAGAGGAAAAACCAGGCTTCCTGCCCAGTAGGAACCCCGATGTG21.7857142857142856No Hit
GTTCCAGAGCTTGCTACTGCTAAGGGTAACCTTTGCCAGGTGATACCTGA21.7857142857142856No Hit
CTTTTCAGGCTTTCTCCGTTGGGTTCCTTTCGTGAAGGCATCTACGGTGT10.8928571428571428No Hit
CCTTATGATTAAAGAGAAAAAGAAAAAAAAAGTTTCCCTGCATAAGAAGA10.8928571428571428No Hit
GGCTGCTGAGCAGTTGTCACCTCCTCCACGAAGCCGTCCCAGCCCAGCGA10.8928571428571428No Hit
CAGGGACATTTTCCCATTACTCCCCATCCCAGGGCATGGGCGGGGTGCAG10.8928571428571428No Hit
GGGGGGGTCAGACCCATTTTAGAGATTTAAAAAACAAACAAACAAACAAA10.8928571428571428No Hit
ACTTTAGTGAAGCTTTCTCTAAAAAGCCTATCTAAAATATCTCCCCATCC10.8928571428571428No Hit
GGGCGCTCACTGCCTCCGTTCTGTAGCCAGCACCAGGCTTAGATCTCCGG10.8928571428571428No Hit
ATGAAAAATTACACACCAACAATATGGATAATCAAAAAGAAAAGTTTAAA10.8928571428571428No Hit
GTAGGGGCAGAGGGAAGGTGGAAGAGGGAAAGGGGAGAGAAAAGAGGAGA10.8928571428571428No Hit
CCCTCATACCCTTTGCAAATAGCCTCCTCCCCAACCACAGCATCAGGCAG10.8928571428571428No Hit
CTAGTTTGCCATGTGAGAGCTGGCTGAAGCAAGAAGGCATGGCATTGCCT10.8928571428571428No Hit
CGTGTGCGAAGAGACGGAAGCGGGGCTGCCAAGGAGACAGGAGGGGAGGA10.8928571428571428No Hit
CATATTCTCGTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATT10.8928571428571428No Hit
TCTGAAAGACACTCATCCAGAACCACAACCTTTCGTGCCCCAAGAAGAAA10.8928571428571428No Hit
GTCATGGGGTCGGTGAGCATGACCCAGGACTAACACACGGGTGGGTCCTG10.8928571428571428No Hit
GCCCTGGCCAGGGGCCTGCCTCCGACATCTCCCCCGTTTTTCTTTTATGG10.8928571428571428No Hit
AGCACAATCTGAGGCTGAAGAGAATCTGAGCTGGGCACAGTGAGACAGAA10.8928571428571428No Hit
CCAGCTCCCCAGAGACCTGGAATCCAGTCCTGGTTCCTCTAATCCAACCA10.8928571428571428No Hit
AGAGGGAAGGGATCCGAGTCAGGGAAGCAATGTAGACACGGAAGCAGAGG10.8928571428571428No Hit
CCTGGGTATTTTCCCCAAAGATACAGATGCAGAGAAAAGAAGGGCCATCT10.8928571428571428No Hit
CCTTTCTTTTCTTTGTTTCTGTGAGTAACAAGGAGGTGATCACAACTGAC10.8928571428571428No Hit
AACGGAGACAATATACACAGTGCTTAGTACAAGTACTTGTTGGTAGTAAC10.8928571428571428No Hit
TCCCAGGGCTACAAACATGCCCAATTTGAAACTATAATTCCATTTCCTTT10.8928571428571428No Hit
GAGAGAAGCAGGCTCCCCGCTGAGCAGGGAGCCTGATGCGGGGCTCGATT10.8928571428571428No Hit
GAGGCGGGGGTGTCCCGGGAAACAGGGACATTTTCCCATTACTCCCCATC10.8928571428571428No Hit
TCCCTACTTCTCTCTCCTGCCCTGATCCCTACTCTGAGTGTGTGAAATGG10.8928571428571428No Hit
GTCATGGTTAGACTGGTCTCCGTATTCTGTGAAAGGGCTTGCAATGTGGG10.8928571428571428No Hit
GGCAGAGGGAGAAGCAGGCTCTCCACAGAGCAGGGACTCCACCGTGGGGC10.8928571428571428No Hit
TAGTCACACAATGATTTTCCCTAAGTGCTTTTCTATATGTCAAATGCTAC10.8928571428571428No Hit
GCCCCTCGGAGAAGGGGATGCACTCACCTCTTTGCTTGGGCTCTGAAGCA10.8928571428571428No Hit
ATTTATCAGGAAGCGAGCCCAGGAGCTACTGCTTCAGAAGGTGAGGAGTA10.8928571428571428No Hit
GTGGAAGGTGAGCAGGCAATCAGGCTTACTGGACACAAATATCTACTCTT10.8928571428571428No Hit
CTGGGAGGCTCAGGCATACCCCTTGGCCAGAGGTTCCAGAAGCATGACCA10.8928571428571428No Hit
CTATTGAAGGTCCCAGTAAGATTAAATCATCTTCACTGAGGGAGTTGGGA10.8928571428571428No Hit
GGGTAGGGTTGGGTAACTGCCGGATCCGGGGTGTTTCATCACTTGTTTTC10.8928571428571428No Hit
GCAGGGAGGTGCCCACCACGAATGTCCAGATAGTAAGAGACCAGAGGGGA10.8928571428571428No Hit
GATTCTTAACAAAGTACACAAGACCTTGGACTCTGAGGAATTCAGGGAAG10.8928571428571428No Hit
GGGGTCACCACAGGGAGACACAGAGATCTGTCATGAAGATTCCTGGCTTG10.8928571428571428No Hit
CTTGCTGCTCGGGCGGGCTGCAGGGAGGAAAGGATCCCTCTCCTGCGTTC10.8928571428571428No Hit
GTTTTGAACCACTGACTTCTCTCCTGAGCTCCTGATCAGTTGCCTTGGCT10.8928571428571428No Hit
TGTGCATACCTGCTGGGCTGCCCAGTGCTGCTGCTGTTCCCATGGCTGAA10.8928571428571428No Hit
GAAAACACCCCGAGCGTGCCCCCGTGTCCGGTTCAGCCATGGGAACAGCA10.8928571428571428No Hit
CTTACGGCTCCTCAGCAGAACCCCAACAAGTTTTGCTTTTGCTGGCCCCA10.8928571428571428No Hit
TGGTAGCTCTGATCATTTTCAGTGTCATTGACACCTCCGTGCCATTCGCT10.8928571428571428No Hit
GCCCATTGGAAGGGTGCAGGCCTTAGAAGTTTCTGCGGGATGGGGGGCCA10.8928571428571428No Hit
GTCCAGGCCCCTGGGTTGCTCCCTTAGACTAGTGCCGGCATCTCCTCCCT10.8928571428571428No Hit
TTGTAATATAATATCACATTCAGCTTGTTGACATTAATACAGTACACCAA10.8928571428571428No Hit
CCTCCATAGGCTTCACAGCCCCCCTTTATCTGGATTGCCAGTTCAGGAAG10.8928571428571428No Hit
GCTAAAAGGAACTGAAATATATAATCATTATGCTATTAATTGGTAATCCT10.8928571428571428No Hit
GCGTACTTAAGAAACATTAAGGAGATCAATATGGGGCTAGAGTGGAGTGA10.8928571428571428No Hit
ATGACATCCTTAAAAAAAAAGAATGCCCAATGATAAGCACATTATAGCAC10.8928571428571428No Hit
GGGTAACCTTTGCCAGGTGATACCTGATGAGATTTGTCACCCCAGGGCTT10.8928571428571428No Hit
GGATGGTAAAACATCTTCAGCTTCCAGACCCCTTCCTTTATTACCGGTCA10.8928571428571428No Hit
GGCGCTCCTCTTCAACTTTTTTTTTTTTTTAAGTAGGCGCCCCCCCCAGC10.8928571428571428No Hit
GGAGAGAGAGAGACCCGGGAGGGGGGCGGCGAGGGGGACAGCCAAGAGGA10.8928571428571428No Hit
CAGGCTCCCTGCTGAGCAGAGCGCCTGCTTCGGGACTCGATCCCCGGACC10.8928571428571428No Hit
ATCAAGAGTAGGAAGCTTAACATAATGAGCCACCCATCCTCCCCTTCCAA10.8928571428571428No Hit
GCTGTCTACACCGCCCGGGTAGCCCCCCCACGGACCACTACTGACTGATC10.8928571428571428No Hit
GCTCTAAGAGTGAGGGGAGGACTGGGCAGAAGATGTGGACATGCAAAGGT10.8928571428571428No Hit
GGACAGTGGGATAACATGCACTGATCAAAAATTAAAAAGCAAAAAAACCC10.8928571428571428No Hit
GCTGTCCCACAAGGTGGGGGCTCGCACGAGATGGGCCCCCTCCATCCGGG10.8928571428571428No Hit
GATGAACATGTGGAGATATGGGGAAAGTGATGCTCCTGGAGGTGCCCTGG10.8928571428571428No Hit
GGTGTGCATAACTGCTGGGCTGCCCAGTGCTGCTGCTGTTCCCATGGCTG10.8928571428571428No Hit
TCACCAGATGGAGGAAACTTCGAGTTTTTACACATGTAGATTGTGTACGA10.8928571428571428No Hit
GTCCCTAAGCTGTGCTATTTCAAAGTCCACCAGGAGGGGGAGCATCTCCA10.8928571428571428No Hit
GGGGGTTTCCCAGTAGTGTGCCCCCCCTGTCCATAGCTGGAGAAAGTATT10.8928571428571428No Hit
CCTGTGGCCCCGCAGGGAGGTCAGAGCCTGAGATCGCCAGCGGAGAGCAA10.8928571428571428No Hit
GACACACACACCATCCGCTTCCTCTCTAATGACACCAAGCAAATGCTCAC10.8928571428571428No Hit
GTACACAGGTGCCCCTGTGGCAGTGTGTGGGAAGTGTGCATGGCAAGCGT10.8928571428571428No Hit
CTTAATAGACTGAGCCACCATGGTGTCCTAAAAAATTAATTTTCTAAAAT10.8928571428571428No Hit
AGCAGGCTCCCTGCTGAGCAGAGAACCCTATGCGGGGCTTGATCCCAGGA10.8928571428571428No Hit
TTCCAGCTCCATGGCCAAGGAAACAGACTAAGGCCAAGGGCTGATCCCAC10.8928571428571428No Hit
TGGAAGGACCACAGTGGGCAGGAAGCCATCTGCAAGCCACGAAGACAGAC10.8928571428571428No Hit
AGTGCACACCCTGCATCAGTCAGGGCTTGAGCCACCTGGGACCCTGAGCA10.8928571428571428No Hit
GTGGCTGATGTTGCCATGCAGCAGGGACCGGCCGGGCCTGTGCCTTCACT10.8928571428571428No Hit
NGCGGAATGGCTGTGCTCAAGGGAGGCGGGATCAAGGAGTTGGAACAGAA10.8928571428571428No Hit
CTTCAGAACATCACAGGGGAAAATCCACGCAACCGGACACGTGTAGCTCA10.8928571428571428No Hit
GTATTTGTGCCAGATGGTGTGGGGATCTTCAAGGGAGGAAAGGAGTCGGC10.8928571428571428No Hit
CCCTAAGACTTCCAAAGAAGGAGGCTGGGAGGTTGAATCTCTAGGGAGAC10.8928571428571428No Hit
GCTTTTCAGCTTGGCGCGAGCCGCACATCGCTGCTGCTTCCTCAGCAAGG10.8928571428571428No Hit
CAGATGCCGTCTGCCTCCAGGATGTGGCAGCATTGGCGGAGGCGGGGGTG10.8928571428571428No Hit
GAACAGGACACAGTCACTCGGCCCAACACTGGCCAGAGCTCCCATGAGTG10.8928571428571428No Hit
CCCCGGCTCCAAGTTCACATCCTGGGTTCGTCCCGCCACGCCAAAATAAG10.8928571428571428No Hit
GGTTTTTATTTTTTTTTTATTTTTTTTAAAGATTTTATTTATTCATTTGA10.8928571428571428No Hit
AGCTGACAGAGTCTCAGCCAGATTCAAAGAAAGAGGACTCATCATCAGAA10.8928571428571428No Hit
GCTAAGATGCTAGACGGGGCCTCTATGTCATAGCTCAGAGAGCTATGACA10.8928571428571428No Hit
ATGTGGGTCAGTGAAGGAGCGGAACAAAAATTGACACGGGGACAGGAGGC10.8928571428571428No Hit
CCCTAGGATGGCGTGGCCCCTCCCAGTAATCAGAAGCTCCCAGGAGGGGT10.8928571428571428No Hit
TGTGTGTGTTGGGTGGTGTGGCTGCTGTGTGTGTGTGTGTGTGTGTGTGT10.8928571428571428No Hit
GTCCAAGATCTCACACCTTATAAGAGAGTTGGAACTCTTCCCTGTGCCAA10.8928571428571428No Hit
GTATGATGGAGGTGAGAGTATGGGACAGGGATTATAGTTATGGGAGTGGA10.8928571428571428No Hit
GGGTACGAATGCATTTTTAGGACTTGGACAAGTTGAGTGTGAGAATATGA10.8928571428571428No Hit
CCATGGGAGGGGCCAGACATACTCAAGGCAACTGTGCCATACCCACGTGT10.8928571428571428No Hit
GGCGGAATGGCTGTGCTCAAGGGAGGCGGGATCAAGGAGTTGGAACAGCA10.8928571428571428No Hit
CTGTGAAGCCTGCAGTGGTTTCCCGTGAAAGGAAATCTGAGGGGCACCCC10.8928571428571428No Hit
GTTAAGAGGTGGTGGCTGGAACTCTGGCCCCGGTCTGGGACCCCACGTCC10.8928571428571428No Hit
ATGTAAGACCTGCCTCTCCTTACATGCTTGCTCTGTGCTAGATGAGCAAC10.8928571428571428No Hit
CGTCTTTGCTTGGGCTCTGAAGCACTTTCTTTCCCGGGTGCCGCAGCTGC10.8928571428571428No Hit
GTGTCAGTACAGCTTGCTTGTCACCCAAACTTAGAAGGACTGGACCCCTT10.8928571428571428No Hit
CCACTGTCCCCTTCTCAAAGGGCCTCTGACCACTTCTCAACAGTATCCAC10.8928571428571428No Hit
GTCCAGGACACTCATCTGCCTGTTCCAGGTACTTCCTGCTGCTCTGGGCC10.8928571428571428No Hit
GCTTTAAGTTCATGATGAACTTTTTTGCTATTTTGCTGTATGGATAAAAG10.8928571428571428No Hit
GGGTGATCCATGTCTTAGTATCAGGCCATCTAGAGCAGTAATGAGCCAGG10.8928571428571428No Hit
GCCTAAGCTGTGCGAAGAGCACCTCAAACACCTGGAGAGGAAGATGATGG10.8928571428571428No Hit
GGGAGACGCAGGCTTCTTAATGAAGGCCTGATCCCCGGACTCTGGGATCA10.8928571428571428No Hit
GGGCATTAGTGCACATGACCACAACGGCAGTGCCTCCGGCCCAGGTGGGG10.8928571428571428No Hit
ACGCTAAGCTGTGATGTGCAAGTGTTTTCTCTCGAGTGAATAAACATTGA10.8928571428571428No Hit
GGGTGACGCAGCTGCCGCAGCCTACAGTGTCCGCATGGAGTTAGAAGGAG10.8928571428571428No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers