FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1966_lungLL_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1966_lungLL_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1550
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC50.3225806451612903No Hit
TGTTGAAGGAGTTCTTGGGGAGCCCCCGGTCTGACAGCCTGTTGTAGTTC40.25806451612903225No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT30.1935483870967742No Hit
GTAAGTGGGGAGACCAAGCACTTGAGAGGCTAAGGGTCTTCTCCAGCAAG30.1935483870967742No Hit
GCTCTTAAGAATGTACTATGAACAATTACACCCCAACAAGCTGGATAATC30.1935483870967742No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT30.1935483870967742No Hit
ACCCCACACTGGGCATAGAGCTCACTTTATTTTTTAAAGATGTCATTTAT30.1935483870967742No Hit
CAGTAACACTATACCAGAGGTTGGAAATGCTCCCGTTTAATGTTGGGAAA20.12903225806451613No Hit
GTGATGTGCAAGTGTTTTCTCTCGAGTGAATAAACATTGAGTGACTAAAC20.12903225806451613No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC20.12903225806451613No Hit
TGGGCACACTGTCCACCATCCTCACTCTGGGAACACCTCTTACAACTTTC20.12903225806451613No Hit
GTCTTGGCACAAGAGTTGGCCAGGATGGGCTCCAGGGGGGCCGTGGGAGA20.12903225806451613No Hit
ACCTACTAAGATTGAATTCTAAAGAAACAGACATCTGACTGTTCAGCTGT20.12903225806451613No Hit
GCTCTAGGCTGGTCCCACTGTTGGGAAGCACAACAGGAAAAGGAAAGGCA20.12903225806451613No Hit
GTAGAGGGATGACCTCATGGTCCAGGTGACACACCTGCAGGGAGGAGACA20.12903225806451613No Hit
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG20.12903225806451613No Hit
TCCTAGAAGAGGGATGTTAGGAGACATGAGCCATAGGGTCCTAAGCGGTC20.12903225806451613No Hit
AGGTGCAGCAGCTCCTTGGCCACCTGTCCAGAGGGCACACGTGGGATTGC20.12903225806451613No Hit
GGTTTTTGTCCCGGGTTTGCTGTTCAATAGATTCCTCGCCTGGGCAAGCC20.12903225806451613No Hit
TTTAATGACTTGTCTTGTGGCCTTACATATGATCTAAGGTGGAGAACGTT20.12903225806451613No Hit
GCCTGCTGCTCCTTCCACTTTGCTCAGTCTGTCTGTCTCTCTCCCCAGCT20.12903225806451613No Hit
GCCCTGGAAGGTGTCGGGCTTGAAGACACCATCGCTTAGCTTGTTGTGCT20.12903225806451613No Hit
GTATTAATTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT20.12903225806451613No Hit
AGTCAGTGGAGCTCTGTGATCCTGATCTTGTGGTTTTGAGTTTCAACCCC20.12903225806451613No Hit
GTTCAATAGATTCCTCGCCTGGGCAAGCCACTTCCTCCCTCCAGGACCCC20.12903225806451613No Hit
GAAAGAGGACTCATCATCAGAAATGAGAGAGATGTTCCCTGAAACCACTG20.12903225806451613No Hit
AGGTTGAGCTGCATGAGGTTCTTGAGGCCCTGGAAGGTGTCGGGCTTGAA20.12903225806451613No Hit
GACTAAACAACATGCTACTGAACAACCAATTGGGTTCATGGGCAAACCAA20.12903225806451613No Hit
TGAGAAAATCATCCTGTTCCTAGAAGAGGGATGTTAGGAGACATGAGCCA20.12903225806451613No Hit
GGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCACTCGGTCT20.12903225806451613No Hit
GACCTAGCCTGCTCTGGTGAGGGTGAGCCATCCTGGCAGAAGGCACACAT20.12903225806451613No Hit
GGAGGAAGGGAGTTCCCATTCTCCATCACAAGGGAGTGTCCTTCACCAAA20.12903225806451613No Hit
GAATTAATGCAGTACAATTATCCAACATTTATAAATATTTACTGCCCCAG20.12903225806451613No Hit
GTGTTGCTGTGCGACGTGGCCACTCCCTCTTCGGGCCTGCTGCGCCCCAC20.12903225806451613No Hit
CACATAACCCCGGGGGGGCAGGGGGAGGCCACGGCAGATCAGGAAGTCAC20.12903225806451613No Hit
GGGATGTCTCGATAAGGGCCGTGCTGGCCTCATAAAATGAGGTGGGGAGC20.12903225806451613No Hit
TTTCCGTTCTTTCCAGGACCTCCGCAAGCCCTGCTCTGGAAGTCAGGAGA20.12903225806451613No Hit
GGTCATGATCTCAGGGTCTTGGGATCGAGTCCCACATCAGGCTTTCTGCT20.12903225806451613No Hit
GAAGTGGGAGGAGCCAGTAGAGGGATGACCTCATGGTCCAGGTGACACAC20.12903225806451613No Hit
GGAGGAGACACAGGAAGTTACTAGCCTGCTGGACCTCTGGAGTTAGAGGC20.12903225806451613No Hit
TTCTCACGCTGTAAGTGGGGAGACCAAGCACTTGAGAGGCTAAGGGTCTT20.12903225806451613No Hit
GGGGAGGGAGGAAGCCTCCCGCCACCACAGAGCTCCCGGCTTTCCTCTCC20.12903225806451613No Hit
CTAAGGACCCTTCCAGATGGACCGCTTAGGACCCTATGGCTCATGTCTCC20.12903225806451613No Hit
GGGGACACAGTCTGAAAACAACTGAATTGTGGGATCTCTAAGGACCCTTC20.12903225806451613No Hit
GTATTTGTGCACTTCAGTAAGCTGTTTACATCACCTAATGAAATTTCAGT20.12903225806451613No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)