FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1966-lungUR-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1966-lungUR-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences680608
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC604.9386884E-448.3437921
GTTACGT803.728619E-545.3223081
TTACGTG1002.7315436E-643.503022
ACGTAGC907.480096E-540.277615145
GTGATCG1001.3910155E-436.249859
GTATTAG1652.3809298E-630.764231
TAATATG2252.8123395E-829.0020124
GTATTAT7950.028.2765561
ACCGTGC1306.480958E-427.8845028
CCGTACA1050.00770413827.6209662
CGATCTA1050.00770690127.6189379
TATACTG3503.6379788E-1226.930445
AGCGAAA3057.075869E-1026.15321
GCGAAAG3108.56744E-1025.7275922
GTATAAC1701.0293038E-425.5937731
ACACGAC1450.001226069824.999899145
TTAGAGT3652.1827873E-1023.837274
CGAAAGC3402.4992914E-923.457513
GCCTAAT1550.001805623423.3921591
GTAGTAT2502.2731601E-623.2050191
Produced by FastQC (version 0.11.5)