FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1966-lungUL-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1966-lungUL-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058074
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGCG451.193896E-464.444389
CTATATA4500.053.166612
GCTATAT3850.052.7346951
TATATAC5200.047.4037973
AGCGAAA5700.045.795921
GCGTATG657.3316914E-444.6216661
CTTCGAC855.3417367E-542.647023
GCGAAAG6400.039.64842
GTCTTAC6350.037.6824721
TATACTG4300.035.4069445
ATGCGCC850.002742070534.1176157
ATATACC5550.033.963934
TTCGACA1301.6548833E-533.4615024
CGAAAGC7650.033.1699033
GTAAGAC5500.031.6363323
TATACGG1453.490607E-529.9999735
GCCTATA1753.7707505E-629.0040861
GTAATAT4150.027.9557461
TCTAGCG1050.00770868827.6190222
TTACGAC1050.00770868827.6190224
Produced by FastQC (version 0.11.5)