FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1966-lungMR-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1966-lungMR-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1255970
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC15340.12213667523905826No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG15070.11998694236327301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1451.9239451E-840.011011
GCGAAAG4100.030.063262
TATACTG6800.027.7215925
GTATTAT16050.027.56211
GTATATA5200.026.4976751
AGCGAAA4200.025.8999821
GTGATCG1751.256006E-424.8570549
CTAATAC4101.8189894E-1224.7569943
GTATAAG3001.7029379E-824.1733171
CGAAAGC5200.022.3085023
GTAATAT5001.8189894E-1221.7559851
GTATAAT4006.87578E-1021.7559831
GTTGACA10150.021.434471
GTCCTAT15600.021.3840891
TTACGTG2755.2089854E-621.0925142
GTCTAAC2104.3069746E-420.7199861
TATTATA6100.020.2064552
GGACCGT2205.9030775E-419.7734436
CGCTTAC1850.00503955819.5999871
TCCTATG16750.019.4790922
Produced by FastQC (version 0.11.5)