FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1966-lungLR-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1966-lungLR-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556527
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2750.058.0257031
TATCGCT400.006047479354.379555
GCGAAAG3450.046.2357522
GTGATCG1151.4111356E-744.130169
CGAAAGC4500.038.6699033
CTAGTAC750.00148221138.66993
GGTATGG1555.4795266E-528.0769581
GTATGAG1801.5145428E-424.177381
CCTAACC2451.9081544E-623.6754513
GTCTATG1550.001802830723.3974651
TAGAGTG2802.3368557E-723.3055215
ATAGAAC2502.2745444E-623.201943
GTATGAA2252.6397576E-522.5655521
GTCCTAT4205.0931703E-1122.4504221
TTAGAGT3757.736162E-921.2684464
TATTATA2053.6615506E-421.2212892
GTATACA2053.6615506E-421.2212893
TAGACTA2806.077873E-620.7160195
CTACGTG2104.3110017E-420.7141579
TAAGACT2857.0835304E-620.352584
Produced by FastQC (version 0.11.5)