FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1966-lungLL-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1966-lungLL-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1756123
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG20050.11417195720345329No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT18280.10409293654259982No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTT451.194132E-464.444199
GTTACGT2500.037.7095151
GATAGCG850.002742600434.11751145
TAGTACC8100.024.1679484
AGCGAAA6500.023.428991
TACACCG3102.4192559E-823.3883365
GTTATGT10250.023.3473531
TTACGTG4053.274181E-1123.2735022
GATATAT7150.022.3133221
CCGACTA5650.021.8153174
ATATACG1700.00309276421.324664
ATATATC6200.021.0495033
TATACTG6900.021.0156065
TACGTGT4501.3278623E-1020.9455553
GTAGGCT6950.020.8644146
TAGCATG27000.020.4090712
GTTGACA16500.020.2172221
CTACTGA23650.019.927066
GCGTTAA2205.905959E-419.772659
GTATTAT22900.019.6337741
Produced by FastQC (version 0.11.5)