FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1915_d06_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1915_d06_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences802373
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC30200.37638355228802567No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT17370.21648285772327835No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG16900.21062523290290178No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG15490.1930523584417721No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCAGACCATTGACAAGG14070.17535485366531525No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA13370.166630731592414No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC12450.15516474258231522No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT11900.14831007523932138No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA11750.14644062050941395No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC11610.14469579609483368No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC10830.13497463149931516No Hit
CCGCTATATGATGCGATAAAATGCATGAAAACATTCTTCGGCTGGAGAGA10510.130986461408846No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG10340.12886774604828427No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA9770.12176381807463611No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG9420.11740175703818549No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG9220.11490915073164226No Hit
GCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATCCCCTCATTCTAG9120.11366284757837064No Hit
GTATAGCATGGTGGGTTCTTGGGACCATTGGATCTTTACAGTCTCCCAAT9120.11366284757837064No Hit
GTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTC9100.11341358694771633No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC9040.11266580505575337No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT8980.11191802316379042No Hit
CTATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGA8880.1106717200105188No Hit
GGTAAAGGCAGTCCGAGGTGATTTGAATTTCGTAAACAGAGCAAATCAAC8680.10817911370397558No Hit
CAGATACACCTACCGATGCCACAGAGGTGATACACAAATTCAAACTAGAA8620.10743133181201261No Hit
GTTTTTACCCTGTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGC8610.10730670149668546No Hit
GTGTTAACTAGCACTGATTCTGGCCCGTTAATCTCCCACATCATAGACGA8420.10493872550546941No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC8310.10356779203687064No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT8120.10119981604565456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTTAA406.116534E-790.62457145
GCGAAAG1100.079.095472
AGTAGAA2350.077.156141
GGTACTG1450.070.004035
GCAGGTA1450.070.004032
ATATCAC955.456968E-1268.688163
CGAAAGC1300.066.9269263
TAACTAT2250.064.468251
ATGTATA350.003566313362.142567145
AATAACT1300.061.3496863
TTATACT3700.058.787174
TATACTG3600.058.4061435
ATAACTA1400.056.9675644
CCCTTAT3950.055.083631
AACACTG3950.055.066465
CGCAATT400.006050564354.374744145
TATACTA3600.050.3501242
AGGTAGG450.00963625348.3361172
ATCACTA1351.9099389E-1048.3361175
AAGTTAC450.00963625348.3361175
Produced by FastQC (version 0.11.5)