FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1915_d04_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1915_d04_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences759349
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG300.001935569172.499935
TATACCG951.0286623E-438.157865
TATACTG3550.036.760535
GTCTACG1102.4337295E-432.958861
ATACCCG1204.0571566E-430.2083055
AGCGAAA2001.06394855E-525.3783171
GCGAAAG2301.1025386E-625.217372
GTATTAC2651.3736826E-724.6258621
TAGTACC3301.7716957E-924.1666454
GTATAGG1550.001806714223.3901581
GTATATA3452.9576768E-923.1189651
TACCTAT3554.12183E-922.464777
GTATAAT2704.4374956E-621.4842911
GTCCTAT6450.021.3593831
GTTATAG2104.3090092E-420.7169971
TTATACT3851.0501026E-820.7142684
ACCTATG3551.0139229E-720.4225168
GTAGCCG2155.054626E-420.2325389
GTATTAG2959.54646E-619.663591
ATAGTAC4904.129106E-1019.2346763
Produced by FastQC (version 0.11.5)