FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1915_d02_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1915_d02_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGGTTGTCCCTGGCGTACACTCTGCTGCCGTGACATGTTTAAGGGTG14.545454545454546No Hit
CTGCTCCCACTTACATGGACGCGAACATAAAATCTGGGAGAGACCTTACT14.545454545454546No Hit
GTTCATTTCTGCACATTAGCTAAAACAGCCACATCTCCCAGTCTTGACAG14.545454545454546No Hit
GATCAACAAAGAGCTGGTTCTTTGAAAAGAACAAAGCTGACAGAGTCTCA14.545454545454546No Hit
AATCAGGTCCTTATGACAGCACCTAGAGTGTTTTACGTTTTTAGAAAGCA14.545454545454546No Hit
CTATTCCTTTTGCCTGGAGTACTTTCTTCTTTGTCTTCCACCCTGCTATA14.545454545454546No Hit
GCATTCGTTGGGAAATCAGAATCGGATGTGTGTGATGCTGCAGCTGAATG14.545454545454546No Hit
CTCAGAGCCTTTGCAGAAAGCCATAGTCAGTTCACCTCCACAGGGAGAAG14.545454545454546No Hit
GGGCACATCCTCACTGGTCACAGCTGTAATATGGGCCAAATCAGTGAAAC14.545454545454546No Hit
GGCCAGCAGCTTCTTGTGATACAGAGGTTTGTTGTACGTTTGTTGTACGT14.545454545454546No Hit
ATAATTGACTCAGCAATTGATTGCTCGTCCCTTCCTCTGACTATAAGTTG14.545454545454546No Hit
GACTACCACTAGGCCGATAGGTGCTGCATCCATGGAAGCAGAGTCTTATA14.545454545454546No Hit
CTCCTCCTGCTTTCCTTTCCCTCCCTTCAGCCACTCTGGCATCCCTGCTG14.545454545454546No Hit
CTCCCACTATGTGTCAAATCAAAAAATGACATCTTCAAAAAAATAAAGTG14.545454545454546No Hit
GGCACGAACAGCAGGCCAGTGGCAAGGAAAGTGTCACCGCTCAGTGTGCG14.545454545454546No Hit
GTCCTAATTGGTCCCAGTTCTCCCACAAAATACTGTGGCAAACTGACAAA14.545454545454546No Hit
CCCCTGCCTCTCCTCTGCGGAGGTGTCCTTCTCCCCGTGCCTAATGCCCC14.545454545454546No Hit
ATAAAACAAATGCACCCATGATATATAAGTGGAAATAACCAGAGAAAGAC14.545454545454546No Hit
GGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATAT14.545454545454546No Hit
CGAATAGAGTGCAGCACTTCTGGTACATTTGTTCATCCTCAAGAATTCCC14.545454545454546No Hit
TCCCTTCCCTGGGGCAGCTCCTGCTCGAGCAGAGGCAAGCACACGGCCAT14.545454545454546No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATATCATTGACGAACG14.545454545454546No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)