FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1915-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1915-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386774
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAGAACATGAATGACATTCAGGTGGCTGATATACAGAATGCTAGCA4780.12358638377967496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1100.072.509311
GCGAAAG1300.066.923022
ATACCGC350.003564862562.1428036
CTACAGT753.755158E-757.999956
CGAAAGC1600.054.374953
GTAATAG553.2144913E-452.7340431
GCATTAA553.2144913E-452.7340431
GCAGTCG657.3310133E-444.6153458
TTATATC907.47396E-540.277743
GTATTAG1209.540287E-636.2546541
ATTATAC1153.1585587E-431.521713
ATTAGGG1402.7423745E-531.0714023
TCTACAG1402.7423745E-531.0714025
TATACCC1652.3814518E-630.757553
TCGCAAG950.004720782430.5302371
GTATAAG1902.0686821E-730.5302371
CTATATG1255.144201E-429.0037211
TACTATA1000.00606994128.9999757
GACGATG1255.148096E-428.999975145
ACCTAAT1050.007697879327.6225951
Produced by FastQC (version 0.11.5)