FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1915-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1915-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences749655
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG1251.2646857E-534.7999239
GCGAAAG3503.6379788E-1226.9374941
GTATTAA6400.023.7969441
TATTAAT5850.023.5469552
TATACAT3554.120011E-922.4647375
TAGAGTG3304.6860805E-821.9696465
CGAAAGC4307.094059E-1121.9185562
GTATAAT2003.0950952E-421.7572061
GCACCGT3004.5518937E-721.749956
TATACTG3355.4942575E-821.641745
GTAATAT2404.3498898E-521.1528381
GTATAGG2404.3498898E-521.1528381
GTTGACA6600.020.8781281
GAATAAC3901.2136297E-820.455491
GTAGGAC5453.6379788E-1219.9540823
AGCACCG3301.1372249E-619.7726825
GAAAGCG1850.005045788419.594559
GTATTAC2608.053346E-519.5256981
AGCGAAA2608.053346E-519.5256981
GGTAGTA2659.321965E-519.1572881
Produced by FastQC (version 0.11.5)