FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1915-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1915-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538041
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG2900.040.0072
GTCTATA800.002029395736.27321
CGAAAGC3200.036.2563443
AGCGAAA3300.035.1740151
GTATATA1655.974289E-835.1740151
TAGCCCG850.002738513834.123625
TATACTG2455.4569682E-1132.5567175
TTATACT1952.599536E-729.7487954
GTATAAG1306.4548256E-427.9024641
GACGATG1306.479439E-427.884314145
CCTTATA2104.9716255E-727.6238822
GTTATAC1658.401468E-526.368253
TAGGACT5500.025.0498354
TATATAC1500.001491856224.1708973
GAATAAC2151.8544524E-523.619761
TATATAA1851.8269678E-423.517632
ATACTAT1952.6012782E-422.3219721
TCTATAT1650.002596672921.9735412
CTATACT3055.3217445E-721.3971884
TACTATA2053.6590738E-421.2232252
Produced by FastQC (version 0.11.5)