FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1914_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1914_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences982642
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG3000.036.251682
AGCGAAA3100.032.755121
CGAAAGC3650.029.7959023
TATACTA2654.5565685E-927.3597585
GTATACG1100.00965254326.3742521
TAGTACT2701.6480772E-724.1677884
CCTATAC2603.2027801E-622.3087253
CTAGTAC2303.1408283E-522.0662383
GTATATA4501.3096724E-1020.9528771
GTACCTA5950.020.7152466
GTTCTCG1750.00365878320.714199
ATATATC5800.020.0009273
TCTAGTA3001.1050495E-519.3342292
TATAGTG5005.4205884E-1018.8508725
GGTTATA3852.3748544E-718.8387531
TAGTACC5851.0913936E-1118.5906054
TACCTAT7450.018.489857
CCGACAA2450.001216941717.75502145
ACCTATG7900.017.4366288
GTATTAC3804.3655436E-617.1779671
Produced by FastQC (version 0.11.5)