FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1914-d12-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1914-d12-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601838
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12290.20420777684360242No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT9680.1608406248857666No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA9320.1548589487536513No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC9320.1548589487536513No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA7860.1305999288845171No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG7240.12029815332365189No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT6990.11614421156523849No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT6100.10135617890528681No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC406.650241E-572.523731
GTGATCG553.2180527E-452.7279
GCTACTA1900.049.605019
CTACGCA750.001482348838.6696782
GTATTAT7600.038.1703831
TACGCAC800.00203519336.2528273
TAGTCGG1251.2642968E-534.7998248
GCAGTGC6000.033.8444061
CAGCTAC2800.033.660547
GCGAAAG1352.1360074E-532.232771
CAGTGCA6400.031.7212222
TATTATT7700.031.073852
CAACCCG2101.4379111E-831.071268145
GTTATGT3350.030.3084261
AGCTACT3201.8189894E-1229.4529748
ATATAGG1255.1415723E-429.0094911
AACCTCG1000.006072714528.99985145
TATACTG2303.4802724E-828.3717755
GTTGACA7150.027.3865811
CGAAAGC1606.820072E-527.189622
Produced by FastQC (version 0.11.5)