FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1914-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1914-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences796377
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC15160.1903621023711132No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT9140.11476976356675293No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC9070.1138907828829813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA2250.038.678561
AGCGAAA3900.037.190921
ACTACGC800.002033294936.2611471
TAGGACT4900.034.0346644
ATTATAC1301.653012E-533.4655233
TATACTG3050.033.2826545
GTAATAT2008.918505E-932.6350331
TATAAGC1402.7437454E-531.0751292
TCAATAC1504.394026E-529.0034523
GCGAAAG5550.028.742162
CCCTGTA3850.028.24657145
CGAAAGC5600.027.1907373
CATTACC5750.026.4814153
CTACGCA1100.00966481326.3667762
TATATAC2507.817471E-826.1031072
ATTACCT7450.024.3317584
TTAGGTA2701.6460945E-724.1695444
TATAATA9800.023.6762892
ACTGTCC6600.023.0680318
CAATACA2552.7031365E-622.7478074
Produced by FastQC (version 0.11.5)