FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1914-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1914-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720516
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGA800.002034922636.2547722
TGTAGCG900.003627691432.221997
GAATAAC3350.030.3088071
GTATTAG1204.0488603E-430.2186011
GCGAAAG3450.029.4241642
TCGCCAT1753.7729023E-628.99979145
GTTATAC1555.4933902E-528.068213
AGGTGCG1555.498589E-528.0643147
GTATTAC1606.809755E-527.1967431
AGCGAAA3800.026.7196061
TAGCGGT1100.00966362226.3671074
CTATATA3151.031367E-925.3207912
CGAAAGC4101.8189894E-1224.7593573
GCTATAT2951.4213583E-824.5846251
TGTTATA3301.7680577E-924.169852
ATATACC2401.5952191E-624.1698474
GTATTAT4900.023.6815171
GTATAAC1550.001804429223.3950461
TATAGAC2252.6475185E-522.5585273
TAACACT4557.2759576E-1222.3106274
Produced by FastQC (version 0.11.5)