FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1914-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1914-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875648
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC2950.036.864363
GCGAAAG3100.035.08062
GTACTAA1601.8742721E-631.7241481
TATACTG3300.030.757545
GTATATC1753.7689133E-629.0049361
AGCGTAG1050.00770798127.6190178
GTATATA4000.027.1921271
AGCGAAA4450.026.071851
GTCTATA2401.5953392E-624.170781
ATACCGC1700.003092512521.3235036
TATATAC4501.3278623E-1020.944423
GTGTACA5950.020.717811
TTATACT3951.4113539E-820.189854
TAGTACT2908.247647E-619.9999774
CTAATAC3651.3596764E-719.8629913
GTCATAG3701.5664409E-719.5979291
TATGCTG6000.019.3333115
GTCCTAT9550.018.9822861
TATAGGT1950.006839940318.5897224
TATAGAG2359.198902E-418.5106162
Produced by FastQC (version 0.11.5)