FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1913_d08_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1913_d08_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382654
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAGAACATGAATGACATTCAGGTGGCTGATATACAGAATGCTAGCA4730.12361036340924178No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACT100.0070891823144.999887
AGGCTCG400.006048063754.3749545
AGCGAAA901.3202334E-648.3396071
GCAGTCG604.941251E-448.333298
AACACCG604.941251E-448.333295
GCGAAAG1151.4080797E-744.136161
GAATAAC855.3312033E-542.6525961
CGAAAGC1252.7130955E-740.599962
GTAATAG750.001481295438.6716881
CATACTC750.001482247138.6666343
TAATACA2450.038.4693534
TAGTGCT1209.548487E-636.2499667
ACCTAAT1501.1303164E-633.8377271
TATTATA1102.4333665E-432.9545142
ACACCGA900.003625470432.2221956
CGCTACC900.003625470432.2221958
CTAATAC2051.1346856E-831.829243
CGTAAAC200.00607585128.99997530-34
ATTAGAC2054.0281884E-728.2926582
TACAGTG2156.108039E-726.9767197
Produced by FastQC (version 0.11.5)