FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1913_d06_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1913_d06_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences741921
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT805.855363E-754.3968471
TACGCAT450.0096382748.33325
TATACTG3500.047.6427275
GTGATCG1453.8380676E-1044.999879
TAACCGT1000.006073542428.9999185
TAATATA2553.0213414E-928.4312952
TAGTCGG1306.481466E-427.8845398
GTAATAT3153.45608E-1127.6301421
TTAGAGT4200.027.618974
GTATATC1358.061796E-426.862641
CCATCCG1358.0808665E-426.851778145
TAGAGTG3607.2759576E-1226.1804815
GTATATA2901.1845259E-825.0100441
TTACGTG1751.2553223E-424.8570752
GTATTAT6000.024.1763741
GTATTAC1550.001803830423.3964941
CTAGTAC1550.001808080423.3870323
TAAGGTA3102.4157998E-823.3870324
ATACTGT5000.023.1999366
GATATAC1600.002174659422.6561873
Produced by FastQC (version 0.11.5)