FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1913_d04_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1913_d04_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532145
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC2250.041.8963553
GCGAAAG2350.040.1135332
AGCGAAA2700.034.9234771
TAAGCGG1301.651317E-533.46755
CTATTAA1952.5929512E-729.7572841
GTATATG1555.4784447E-528.0774351
TAGGACT4500.027.393774
AGCGGTC1658.411863E-526.363387
CCATTAC2808.1618055E-925.8974742
AAGCGTG1409.988993E-425.8926057
TATACCG1701.0304727E-425.5879869
ACGTATA1751.2507313E-424.8685861
CGTATAC1751.2531289E-424.8615722
CGGTCCA1751.2547296E-424.8569019
GCGGTCC1801.5190823E-424.166438
GTAGGAC4053.274181E-1123.2757533
GTATATA1600.00216801422.666681
ATATACA2903.2655407E-722.5103571
GTCCTAT3253.961759E-822.3179631
ACACCGT1650.002596586721.9736146
Produced by FastQC (version 0.11.5)