FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1913-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1913-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810389
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC30420.375375282734588No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT17720.21866042110640693No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG17080.21076297926057733No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG15580.1922533499344142No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCAGACCATTGACAAGG14170.17485429836782088No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA13470.16621647134894477No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC12520.15449370610904145No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT12040.14857062472466925No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA11870.1464728667343708No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC11690.1442517112152312No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC10960.13524369160983182No Hit
CCGCTATATGATGCGATAAAATGCATGAAAACATTCTTCGGCTGGAGAGA10540.13006099539850613No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG10410.12845682752357201No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA9850.1215465659084711No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG9480.11698085734135087No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG9320.11500649687989349No Hit
GCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATCCCCTCATTCTAG9190.11340232900495936No Hit
GTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTC9180.11327893147611826No Hit
GTATAGCATGGTGGGTTCTTGGGACCATTGGATCTTTACAGTCTCCCAAT9170.11315553394727718No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC9130.11266194383191283No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT9060.1117981611300252No Hit
CTATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGA8960.11056418584161434No Hit
GGTAAAGGCAGTCCGAGGTGATTTGAATTTCGTAAACAGAGCAAATCAAC8760.10809623526479258No Hit
CAGATACACCTACCGATGCCACAGAGGTGATACACAAATTCAAACTAGAA8710.10747924762058716No Hit
GTTTTTACCCTGTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGC8650.10673886244754063No Hit
GTGTTAACTAGCACTGATTCTGGCCCGTTAATCTCCCACATCATAGACGA8500.10488789951492432No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC8370.10328373163999018No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT8170.10081578106316844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA2200.085.697571
GCAGGTA1300.066.922992
GGTACTG1400.062.142785
TAGGGGT350.003566277462.142784
GGATAGC3800.057.236775
GTGTCGG651.0934666E-555.769157145
TAACTAT2350.055.5421261
GCGAAAG553.2157172E-452.7369651
GTACTGG450.00963148348.342221
AGGTACT2000.047.124944
AACACTG4650.046.7741365
ATAACTA1402.728484E-1046.6070864
AATAACT1402.728484E-1046.6070863
TATACTA3050.045.163882
GTAGCCG657.335801E-444.6153269
GTCTTAA700.00105556741.4361881
GTAGATG700.001056528941.428522
CTAATAG5350.040.6541524
AGAAACA4850.040.3607754
CTATGTT1807.2759576E-1140.277734
Produced by FastQC (version 0.11.5)