FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1913-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1913-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences766338
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG604.9444084E-448.3332252
GCGAAAG2404.3655746E-1133.229092
TAGCGGT1352.1415854E-532.222154
ATAGCGG1000.006073649528.9999353
CGTAGAT1050.007707430527.6189842
AGCGAAA2756.7357178E-926.372181
GTATTAT5800.026.2585071
GTAATAG1600.002170597422.6635911
TATACAG4659.094947E-1221.827915
GGTAGCG2003.1021694E-421.749958
CGAAAGC3757.756171E-921.2666193
GTATAAA5800.021.2568861
GTAGCGG2053.6654182E-421.2194639
GTATTAA7100.019.4076961
GTATTAG2307.942294E-418.9191741
GTAGCCG2307.959741E-418.9130029
TACTATA3501.997876E-618.6428172
CAATTCG2359.197539E-418.5105959
TATTATC3953.1203672E-718.3543912
TATACTA3201.9270135E-518.1249585
Produced by FastQC (version 0.11.5)