FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1913-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1913-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890779
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG9800.11001606459065605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTG4300.052.275865
AGCGAAA3150.050.645921
CGAAAGC3850.045.2020873
GCGAAAG4250.039.241612
GACAGTA4450.039.1008387
TATACTA2350.037.0272375
GTGATCG1905.4260454E-934.3418549
TACTGAT6600.034.0527767
GCAGGTA6850.031.7569392
GTACTGA7750.030.8759386
AGGTACT7500.029.9714934
GGATAGC5100.029.857755
CAGTATG5950.029.2434839
GTCTAAC1255.146847E-429.00631
ACTATAC3301.8189894E-1228.5652053
TTAGAGT3351.8189894E-1228.1388594
CAGGTAC8000.027.191883
ACTGATC8350.026.915978
TAGCGGT1100.0096634426.3678824
ATAGCGG1100.0096634426.3678823
Produced by FastQC (version 0.11.5)