FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1912_d08_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1912_d08_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1181
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAACTCTCATGTTCCTGCATATCTGGTATCAGCTTTGGTTCCCCACT20.1693480101608806No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG20.1693480101608806No Hit
CCTCTGGAGTTAGAGGCCCTGTCTTGGCACAAGAGTTGGCCAGGATGGGC20.1693480101608806No Hit
CATTAGTGTGTGCAGAGCAACTGCCCATATAAAGGAGGCCAGAACGCAGG20.1693480101608806No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT20.1693480101608806No Hit
CCGATCCCCCGCGGCCTCCCGCCTGCTGATGGCGGCCTCTTAAGCGGGTG20.1693480101608806No Hit
GCATGAAGCAGAACGGTGCCCCACTGTTTCCTGGCACTTCAACATCAATG20.1693480101608806No Hit
CCACTGAGCAGGGAGCCTGATACAGGGCTCAATCCCAGGACCCTGGAATC20.1693480101608806No Hit
TTGTATACCCTGCTCCTCCCCCTCGAAAGCATTTGGGGACTCGGCCCCTC20.1693480101608806No Hit
TCAGTCAATAACACATCCCTGGGAATTCATTACAGGAAAGCGAACACACA20.1693480101608806No Hit
GTAGAGCAGTACTGTGTTCCCATTCGGTACCCCGAAGAACCCCGAAGAAC20.1693480101608806No Hit
GCTTAGACCCAGGCTTTGCGTCTCTCCAAGTCCGAATTTAAGGATTAACC20.1693480101608806No Hit
ATTTCATCCCATTCACTTGAAAAACTGTTTTCCAAAAGGTGTGAGCCCCA20.1693480101608806No Hit
GGATCAAGGAGTTGGAACAGAATCTCTAGGTCCAGGTAGCACTTTCTACT20.1693480101608806No Hit
GTCATCATCATCATCATCATCATCATCATCACCATGAACAACAACAACAA20.1693480101608806No Hit
GAAGGAAGGCCTGTCCTGTGCCTCTGGTCCCAAGGGGCAGCAGCTCCTTG20.1693480101608806No Hit
ATGATTTTCTGTGGTGAGGTGGACAAAGAGCTCCTACCCCATTGGGGCCC20.1693480101608806No Hit
GTGCCGAGCTCCCCTGGGGAGTCCTCGCCAGCCTCCTCCTCACGAGTCTC20.1693480101608806No Hit
GATCCATAGAATCCAGTTCCATTTGTGCCCCCAGCTCATTCAGTAACGTT20.1693480101608806No Hit
GTCCCTGGGGACGCCTGGGTGGCTCAGGTCATGATCTCATGGTCTTAGGA20.1693480101608806No Hit
GTGTAAGGACAGTGGCCTGAGGTTTATGCTTTTACCAGAGATGGGGTGGC20.1693480101608806No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG20.1693480101608806No Hit
GCTTCATTCTCTGCCCCATGTGAATAACCGGGCATAGAATCCCGGTTATT20.1693480101608806No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers