FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1912_d06_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1912_d06_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATAACCTACCTCCTTCAGTGGGGGAGGACCACATTTTAAGAATGTGG21.5384615384615385No Hit
GGATTGGGAGGAAAAGGAATGTCAAATAAAGAAGCATGTAGTTCACCATG10.7692307692307693No Hit
TTTGGACACTTAGCAGGAGCCCTCAAGAGCAATATTTTTGAGAGAATCAA10.7692307692307693No Hit
ACCCAATCTCAGGACCCTGAGACCATGACCCGAGCAGATGCTTACCGACT10.7692307692307693No Hit
CCTATAATGTTCTCAAATAAAATGGCGAGGTTAGGAAAAGGATACATGTT10.7692307692307693No Hit
CATTTGACACACACACACAGAGAGATCACAAGCAGGCAGAGAGAGAGAGG10.7692307692307693No Hit
GCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGGTACATTTGTT10.7692307692307693No Hit
GTATGGCTGTGGGCTGTTTGCTTAGCTTTTCTGAACCCCTATTAACTCAT10.7692307692307693No Hit
GATTTCTGGTTGTCCCGTCGCCCCTCCCCAGGGCTCTACTCTGGAATCCC10.7692307692307693No Hit
GTGTTTTTTTATGATATTGTAGTAAAAAAATAAAAAGTTGTTGTAGGAAA10.7692307692307693No Hit
TTCCAGAAGACTGAAACCTTAACGTTAACCAAAATTTTTGCTGACCACAA10.7692307692307693No Hit
GATCTTGAGCGCTCTCCCAAAACAGTATTATAGGTCAAGCCAGTGCTTTT10.7692307692307693No Hit
TTTCTGTTGTCTTTTTAGTTTGTATTTCATTTATTTAAGTTCTGATGTGT10.7692307692307693No Hit
GCCTGACGCAGGACCCAATCTCAGGACCCTGAGACCATGACCCGAGCAGA10.7692307692307693No Hit
ATACTACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTC10.7692307692307693No Hit
ACTAGGATGAATGTTGAGCATTGTCACCACTCAGAGGGCATTTGTCTTAT10.7692307692307693No Hit
ATGTGGGGCTCCATCCCAGGACCCTGGGATCATGACCTGATGCGAAGGCA10.7692307692307693No Hit
GGGTAAAGGCTCTGGGTGAAATGGGAAACTTCCAGAGAGGCAGCTCAAGG10.7692307692307693No Hit
CGAGGGATGAGAACATTAATTACATCCAAAGAACGGATTTATGAGTGACA10.7692307692307693No Hit
GATACACCATTTAATGCAAACATTTACCACAAACTTCATTAAGCAAGAGA10.7692307692307693No Hit
GTAGAAACAAGGGAGCAGAGAATATATTCTGTTGAATTAACAGCGAGCAA10.7692307692307693No Hit
CATATTGGCTTTTGCCTGATTTATTTAGGAAAGGAGGTGAGAGAAAAAAA10.7692307692307693No Hit
GTATTCTAGGGCAAGCCAGTGCTTTTCAGTTGTTTTCACTTTGTAGCACA10.7692307692307693No Hit
GGCAAAGGGAGAGGGAGAAGCAGATTCCCCAGTGAGCAGAGAGCTCGTCA10.7692307692307693No Hit
GATTTGTAGACCACTATAACAATAGAAAATCCTTCGAGACGTGGTTACGT10.7692307692307693No Hit
GAAAGAAACTGTGCAGATCAAATGAGATAAAAACTCTTGAGAGCTACTAG10.7692307692307693No Hit
CCCCTAAATGCATACTTTAGAAAAGAGGAGGGATGCCTGTCTGGCTTAGT10.7692307692307693No Hit
GAATAGGCCACCCAGGCATCCCTCTTTTCTGTTTTCCATTACTACTTTCT10.7692307692307693No Hit
CTATTACCTCATCCATAAAATGGGCCTATGATATAACCTACCTCCTTCAG10.7692307692307693No Hit
GGCCATAGGTTACCAACCTTGTTCCAGAAGACTGAAACCTTAACGTTAAC10.7692307692307693No Hit
ACCCTACCTTGAAAAGGGGCAAATTAGCATCTGGCTAATGTGGGCTCTGA10.7692307692307693No Hit
AGCGAAAGCTGGCATGCTCAGAAGCCTTTCCAGTACAGGGCATTGGACTG10.7692307692307693No Hit
CCCAGCATGATTGTTGTCGCCCCTCTCTGCATCCTGGGCGGTACAGGAGC10.7692307692307693No Hit
GTGGGTTCTTGGGACCATTGGATCTTTACAGTCTCCCAATTCCTAATGAT10.7692307692307693No Hit
GGATGAATGTTGAGCATTGTCACCACTCAGAGGGCATTTGTCTTATATCA10.7692307692307693No Hit
ATTAAAACCAACCAATCAACCAAGCCCACCAATTAAAAAATAACCGCCCT10.7692307692307693No Hit
CTCTGGAACAGCGGGGCAGTGGAAAGACCAATGTCACCCACTCTCTGTAT10.7692307692307693No Hit
GGAAGCAGGCTCCCCGCAGAGCAGAGAGCCCGATGTGGGGCTCAATCACC10.7692307692307693No Hit
CAGAGGAAGAGAGAAAAGGGAAGAAAGACAGATGGAGAGGGGGAGGGAAA10.7692307692307693No Hit
ATATTACTTGGCTGTTACTCGGCCTAATTCTTCATCTTATCACACTGAAC10.7692307692307693No Hit
TGATATAACCTACCTCCTTCAGTGGGGGAGGACCACATTTTAAGAATGTG10.7692307692307693No Hit
AAGGAATCCACTGCAGCAAAGGAAAATTAGTGGTCACCAGGAAGAAAGTT10.7692307692307693No Hit
AAGCAGGCTAGCTTATGAACCTGTATTTGCCTTTCATACTCCCTTGGTTT10.7692307692307693No Hit
CTAAAAAACGCATTAAGGGGTGATCAATTATCGGCCAGAACATCATGGAG10.7692307692307693No Hit
ATATAGATGTTACATTCATGAAATAACATAATGGCATACAAAAGACTGAT10.7692307692307693No Hit
GGTACGGTATCTGCGATGGGATTAGAATTTTCAACTAGGATGAATGTTGA10.7692307692307693No Hit
GTATGATTTGAGCTGCCTGCTGGTACCAAAGAAGAGAGCATTCAGGCTTT10.7692307692307693No Hit
CTGTTTGCTTAGCTTTTCTGAACCCCTATTACCTCATCCATAAAATGGGC10.7692307692307693No Hit
TTACAACCATTAGCAAAAAATGCTTTTTCACCTACATACTGCTTTTCTTT10.7692307692307693No Hit
GATGTTAGGCTTCTGTAGCATTTATCGCTCAGAAATAGTCAAATACCAAC10.7692307692307693No Hit
GAGAAGCAGGGTCCCCACTGAGCAAGGAGCCTGATGCTGTACTTGATCCC10.7692307692307693No Hit
ACACTGAAGTGGTGGAGAAATGGAAGTCAACAATCACAAGTCATAACATC10.7692307692307693No Hit
GTCATGATGCCAGACTTACATAAATTCAAATTTCAAATTACCCATTAAAT10.7692307692307693No Hit
ATGTGGGACTAAATCCCAGGATCCCAGGATCCCAGGATCATGCTCTGAAC10.7692307692307693No Hit
TGCGGGGCCCGACCCCAGGACCCCAGGACCCCAGGATCATGACCTGAGCT10.7692307692307693No Hit
CCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGGGAGGTGG10.7692307692307693No Hit
ACATATATCACTGGGTTAATCTTCTAAACAAAATCAACAAGGAAACAATG10.7692307692307693No Hit
GCTCTGAGGAGGGTGACATGTTTGTAGGAGGGTTTCCCTTTGTTTTTTTT10.7692307692307693No Hit
TCCTTGGGTTCAGCAAGGACTTTTGATCATCCCTGGTTTCAGCAGGCTTC10.7692307692307693No Hit
GGAAAATAGAATTCTCACAGAGCAGGGATCAACCTTTTCCTCCATAAATC10.7692307692307693No Hit
GTTTATAGCATGTTAACAAAAACAAACATGGTTACATTTATAATGAAAAC10.7692307692307693No Hit
ATAACATTCATTTATTAATAAGTCACATAAGTACACATTCGGCATCAAAT10.7692307692307693No Hit
CTGCTGATGGCTGCACGGGAATAAAACAGGGAGATGAAACATTGATTCTG10.7692307692307693No Hit
GGGCTGTTTGCTTAGCTTTTCTGAACCCCTATTACCTCATCCATAAAATG10.7692307692307693No Hit
TATCAGAGGAGGTGAAGCCACACTGGCATCTTATAAGTATATGGGGACCC10.7692307692307693No Hit
ACCCCAGCCAAAGGCAGAGGCTCAACCCACTGAGCCACCCAGGTGCCCAA10.7692307692307693No Hit
GGTCAAGACTCTGTGATAAACCAGAAATGGTCCACATGAAATTTACCAGA10.7692307692307693No Hit
CTAAACAAATTCCAATCAATAAAAGAGACACAGATTCAAGAACTTAATAG10.7692307692307693No Hit
GTACAAAGCATCAGCGTTAGTTTCCAACCAAGGTTATCCTTGGGTTCAGC10.7692307692307693No Hit
TTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCAA10.7692307692307693No Hit
GCTTATGAACCTGTATTTGCCTTTCATACTCCCTTGGTTTTATCATCATT10.7692307692307693No Hit
ACCGACTGACCACCCAGCTGTCCTTTCCTGCCTTTTAAATTAAGAGAATT10.7692307692307693No Hit
CATGGACCTTCTGTTAATCAGGAGACAAAATAAGGAGCATAAGACAAGAC10.7692307692307693No Hit
AAACTTTGTAACCTTTCAGTTTCCCTCAGTTTCAGTGTCAAAACTACATT10.7692307692307693No Hit
GAAACAAGGCTGGTGGAAAAGATTTTGGACACTTAGCAGGAGCCCTCAAG10.7692307692307693No Hit
GAACAAATATAATGTCTGTAGCTTTTTTTGAAAATAAAAACTTTGCTTAC10.7692307692307693No Hit
CGAAAGCAGGGAGCGAAATCTGAGTATACAGAATGAAACCCCCTTCAGAG10.7692307692307693No Hit
CTTATGAACCTGTATTTGCCTTTCATACTCCCTTGGTTTTATCATCCTTG10.7692307692307693No Hit
AAACAAGGGAAGGAAAGGGGTGCCTGCGTGGCTCAGTTGTTAGGCATCTG10.7692307692307693No Hit
GGTTATCCTTGGGTTCAGCAAGGACTTTTGATCATCCCTGGTTTCAGCAG10.7692307692307693No Hit
GAAGAAACAAGGGATGAGGTGTAAAATAAGCTATTTGGTCAAGACTCTGT10.7692307692307693No Hit
TATATGGCCTAGGCACCGAATCCCTGCCATTTCATCATTTCTTAAAGTGT10.7692307692307693No Hit
CTGAGATGATATGAATTGATTCTCTCAAAAATATTGCTCTTGAGGGCTCC10.7692307692307693No Hit
GGCATTACCACAGCATTGTTGACGGATTCAACTTCCTTATGACTGACAAA10.7692307692307693No Hit
ATATTTCAGTTATGAAAGATAAATGCACATTTGAACTCCACTAAGATGGT10.7692307692307693No Hit
GGTTAAATACCTCATCTGAGGCCACGTAGCTGCTCACAACTGAGCAGAAA10.7692307692307693No Hit
ATATTGAATCATCCTCTAACACACTAATTTTCTACAGACTTAGTTATTTC10.7692307692307693No Hit
GAGGAGGGTGGAGAGTGAAGCAGACTCCTCACTGAGCAGAGAGCCTGACT10.7692307692307693No Hit
ACAAGGTACGGTATCTGCGATGGGATTAGAATTTTCAACTAGGATGAATG10.7692307692307693No Hit
CAGCTGTACTCTTGGTTTGCATAGCCCTCCCTCCATGCCACCCCAAACAA10.7692307692307693No Hit
GCTTTTCTGAACCCCTATTACCTCATCCATAAAATGGGCCTATGATATAA10.7692307692307693No Hit
GGGCACCAGGGTGGCTCCGGCAGTTAGATGTCGGTCTTTGGCGCCGGCCA10.7692307692307693No Hit
GTTTTATGACACAGATAGCGTGCCACTGAGGAAAAAACAAACACAGATTT10.7692307692307693No Hit
TCTGAACCCCTATTACCTCATCCATAAAATGGGCCTATGATATAACCTAC10.7692307692307693No Hit
AATTTTTAGTTCATCTTGAAATTTTAAATAAAAAATGACAGATAAGATCC10.7692307692307693No Hit
CCCCTGAACAGATGCTCATTATTTCTCAATAGAGTGATGACCCAGCAACA10.7692307692307693No Hit
AGGATGAATGTTGAGCATTGTCACCACTCAGAGGGCATTTGTCTTATATC10.7692307692307693No Hit
GTTATACAAATGCACGAATGCACAACACAAATTGCGTCTTGTTTTTGGAA10.7692307692307693No Hit
GAATTAAGGTGGTCATTGATTTCTGAAATAAACTATGAAAACTAAATGGC10.7692307692307693No Hit
AAACAGCTCTGATTGACGCACAAAGGCTAACTTCAGCAGTAGAATAAATT10.7692307692307693No Hit
GGACTAAGTAAGATGAAGATCTAGGGGACTGAGCCCAGAGAGGCAAAGTG10.7692307692307693No Hit
GAGATAAGGAGAGGCAGGCTCCCTGCTGAGCAGAGAGCCTGATGCAGGGC10.7692307692307693No Hit
GACTGAGCCACCCAGGTGCCCCTATCGGAACATTACTTCTGATGCACCAA10.7692307692307693No Hit
TCTTATAAGTATATGGGGACCCACTTTCTTCCTGGTGACCACTAATTTTC10.7692307692307693No Hit
ACTATATTGGCTTCTGGCAGCCTTTGGTACTAAAGATTGAAATGGTTCAA10.7692307692307693No Hit
GAATGTTGAGCATTGTCACCACTCAGAGGGCATTTGTCTTATATCAGAGG10.7692307692307693No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT10.7692307692307693No Hit
GGTTCTAGAAACAACCATTATAAATTGGAAGAGTTTTTATTGGTCCCCTG10.7692307692307693No Hit
CCCCCATAGAGGTATTCAGATCGAACGGTCTAACAGCCAATGAGTCAGGA10.7692307692307693No Hit
AAACAAGGATCATTCATTCTTACAGCTAATATTTACTGAGAGTCTACTCC10.7692307692307693No Hit
TCATCACCCTCAGAAGGAATTTTGACTCTTGGGTGAGAATTATTCCTAAA10.7692307692307693No Hit
ATTATATACAAATGTTGCATCTGCAAGATCCATTGGACATGGCCCAGAAC10.7692307692307693No Hit
CTCTCTCTGCCTGCCTCTCTACCTACTTGTGATCTCTGTGTGTGTGTCAA10.7692307692307693No Hit
GTACTATGTTAAAATTTTTAATATATATCTAATAAAAATGTAATACATAA10.7692307692307693No Hit
AAAATAAGCCTCCCATTAGGGATCAAAGCTCAAAGTCCCAATCCTGCAGT10.7692307692307693No Hit
AGCAGGGGGAGGGCAGAGGGAGAGGAAAACAATCCTCAAGCAGGGAGCCG10.7692307692307693No Hit
CTTATAAGTATATGGGGACCCACTTTCTTCCTGGTGACCACTAATTTTCC10.7692307692307693No Hit
TCATGGAGCTGGGAGGTGGGGCCGTGAACCGCATCCCCACAGGCAGGTGT10.7692307692307693No Hit
AGCGAAAGCAGGAAAGGCACGAGGCAGCAGAGGTCTGGGCTTGTCCTGGC10.7692307692307693No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC10.7692307692307693No Hit
TCCCTTAGCTTTCCGGGTACCAAGAAATCAGTGACCTAGGGGCGCCTGGC10.7692307692307693No Hit
GTATCTGCGATGGGATTAGAATTTTCAACTAGGATGAATGTTGAGCATTG10.7692307692307693No Hit
GGACAGAGGAGAGAAGAATCCCAAGAAACGGGGGTGGAAGTGAATTACAG10.7692307692307693No Hit
GTACGGTATCTGCGATGGGATTAGAATTTTCAACTAGGATGATTGTTGAG10.7692307692307693No Hit
GTTGAGCATTGTCACCACTCAGAGGGCATTTGTCTTATATCAGAGGAGGT10.7692307692307693No Hit
AGCGAAAGCAGGGTCAAATCCAGGTTTTGCCTTTGCCATTTATTTTCTGT10.7692307692307693No Hit
GGCAGAGGGAGAGGAAAACAATCCTCAAGCAGGGAGCCTGGTGCGAGGCT10.7692307692307693No Hit
CCACTGAGCCACCCAGGTGCCCAAGGATTCTTATCTTTAATTTGGACAAA10.7692307692307693No Hit
AAATAAAACTAGAGCAGCAAAAGAAGGTATGTTGCCAAATTTCTGAATTA10.7692307692307693No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers