FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1912_d04_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1912_d04_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1145
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCCGTATTGTAGAATTTGGTTTGCAGATATTTGGGTGAGGATTTTTAT30.26200873362445415No Hit
GGATATTGTGGGCCAGGTTGAGCTGCATGAGGTTCTTGAGGCCCTGGAAG30.26200873362445415No Hit
TATATGAACAAACCTAAGAGAAGCAATCAGCACCAATATCATAGGGAACT20.17467248908296942No Hit
GAGCTTAAATAAGAAATCAAAAACTTCCAACAAGCAAAAGTCCAGGACCC20.17467248908296942No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA20.17467248908296942No Hit
GAGCCAAAGGCAGCGGCTTAACCCACTGAGCCACCCAGGTGCCCTCTTGT20.17467248908296942No Hit
CTCACTAGCTGCGTGACCTTGGGTAAATCACTTAACCTGGTACTAAGCTG20.17467248908296942No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA20.17467248908296942No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT20.17467248908296942No Hit
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC20.17467248908296942No Hit
GCGTAGGGATGGCCTGCCCTTTTGCTCTGGCGTTCCGGTGGACAGGGGAC20.17467248908296942No Hit
GTGTGAATGGAGTGCTAAGAGAAGGGCTGAGTGCAAAGCACCCACCACAC20.17467248908296942No Hit
CCTCATGGTCCAGGTGACACACCTGCAGGGAGGAGACACAGGAAGTTACT20.17467248908296942No Hit
AAATTAATACAATGAAACAAAATTCACAGAATGCAGTAAAACAGGTTCTA20.17467248908296942No Hit
CAGCCACACTGACTCTGAATTATGTTTACAAACTAAATAAGGTAGTGCAT20.17467248908296942No Hit
AAAGCAGGCTGGCCCTGGCTTAACCACAGGGAGACACAGAGATCTGTCAT20.17467248908296942No Hit
GGCAAAGACCAGGTGAGCCGAGGTGTGAGGAATATTCCAGAATATTCCCA20.17467248908296942No Hit
GTAAGAGACCAGAGGGGATGGCTGTGCCTCCAAATGTGCCCTGCACAGGC20.17467248908296942No Hit
GCCTCAACTGCTCCAGGTGCCGGGGCAGGGCCGAGGGCACCTCTTCGAGC20.17467248908296942No Hit
GCCAAGGAGACAGGAGGGGAGGATTGGGCAGGGGATCGTTCTCCACTCTG20.17467248908296942No Hit
GACTTGGAACATAGAAACTTAAAGAAAATCTTGGCCAAAGTGCCGTCCCT20.17467248908296942No Hit
CTTGAGGCCCTGGAAGGTGTCGGGCTTGAAGACACCATCGCTTAGCTTGT20.17467248908296942No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA20.17467248908296942No Hit
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC20.17467248908296942No Hit
CTAAGGACCCTTCCAGATGGACCGCTTAGGACCCTATGGCTCATGTCTCC20.17467248908296942No Hit
TCTCTACACAGCAGATTTAAAAATACAGAGTGCTAAGGTTGGCACAGGGG20.17467248908296942No Hit
TCCCAGGGCCGCCCACTCGGTCTCCCTCCCTCGGGGGACTCCACAGAACT20.17467248908296942No Hit
AAAGTCCAGGACCCCATGGTTTCACCTGTGAATTCTACGAATCATCCATA20.17467248908296942No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTTTT350.00322251320.714287125-129
Produced by FastQC (version 0.11.5)