FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1912-rep2-d12-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1912-rep2-d12-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1119230
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG11370.10158769868570357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACA9450.028.3949741
GTTACGT2405.2570613E-827.1958641
CCGACTA3151.033186E-925.319594
TTACGTG2601.1440716E-725.0982632
GTATCGG1801.5172745E-424.1740991
GTATATA5100.024.1740991
GCATCGA2252.6520145E-522.555437145
ACATGGT11700.022.3095665
GTAATAT4006.87578E-1021.7566911
AGGTCGG5051.8189894E-1221.534548
GCACCGT4409.822543E-1121.4222566
GATGCGC2053.666948E-421.21948
GTATTAT16100.020.7206571
AGCACCG4652.055458E-1020.2705235
TTATACG2908.251307E-619.9998959
CACCGAC4402.2610038E-919.7743912
ACCTAGG2959.53852E-619.6670651
CAGGTCG5555.456968E-1219.5944927
TACACCG2608.073192E-519.5208725
GGTCCTA16150.019.3033059
Produced by FastQC (version 0.11.5)