FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1912-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1912-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757728
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGTT951.9203235E-645.789277
AAGGTCG1151.4112993E-744.133155
TATACGG750.001482711338.6690445
TATACTG5250.037.2880065
TAGTACC2052.910383E-1035.3680274
GTGATCG2103.783498E-1034.523659
TTATACT4500.033.8354154
ACGACTA900.003626588532.22425
GCGAAAG4150.031.4477142
ATATACG950.0047251630.528194
TACGGAG1504.3954748E-529.0017835
CCTTACG1255.150822E-429.0017812
AGCGAAA4900.028.1213931
TGCACCG1050.007704980727.6207435
CGTAGCC1050.007707462627.6189219
AGGTCGT1606.8231326E-527.189176
AACCGCG1606.8261994E-527.1873787
TATAGAG2156.118007E-726.9784035
ATTCGAC1907.1343766E-626.7192211
GTATATA2756.7357178E-926.372221
Produced by FastQC (version 0.11.5)